Quantitative determination of incarvillateine in Incarvillea sinensis by solid phase extraction and high performance liquid chromatography
| Autor: | Motoyuki Nakamura, Yu-Ming Chi, Wen Mei Yan, Yu Cheng Chi, Toshihiro Nohara, Toyokichi Yoshizawa, Fumio Hashimoto, Junei Kinjo, Xi-Ying Zhao |
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| Rok vydání: | 2005 |
| Předmět: |
Detection limit
Chromatography Chemistry Analytical chemistry Reproducibility of Results General Chemistry General Medicine Reference Standards High-performance liquid chromatography Quantitative determination Incarvillea sinensis Solutions Structure-Activity Relationship Alkaloids Drug Discovery Incarvillateine Bignoniaceae Calibration Monoterpenes Sample preparation Solid phase extraction Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Chemicalpharmaceutical bulletin. 53(11) |
| ISSN: | 0009-2363 |
| Popis: | Method for rapid quantitative analysis of incarvillateine in Incarvillea sinensis by high-performance liquid chromatography (HPLC) has been developed. The sample preparation involves solid phase extraction (SPE) with a mixed-mode reversed-phase and cation-exchange cartridge. The linear calibration range for incarvillateine was 0.002-0.5 mg/ml. The limit of detection was 0.35 microg/ml (S/N=3). Intra- and interday precisions were less than 0.36% (n=6) and 1.61% (n=18), respectively. The recovery of incarvillateine was 97.61-102.44% with the relative standard deviation (RSD) ranging from 0.63 to 1.93% (n=3). This method was proposed as a simple, rapid and accurate method for quantitative determination of incarvillateine content in various samples of Incarvillea sinensis collected from different areas of China. |
| Databáze: | OpenAIRE |
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