Versican is differentially regulated in the adventitial and medial layers of human vein grafts

Autor: Steve P. Evanko, Michael Sobel, Mark W. Majesky, Thomas N. Wight, Jacques-Antoine Haefliger, Tannin A. Schmidt, Marco Piola, Richard D. Kenagy, Alban Longchamp, Shinsuke Kikuchi, Matthijs S. Ruiter, Monica Soncini, Sébastien Déglise, Maurizio Pesce, Gianfranco Beniamino Fiore
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Genetics and Molecular Biology (all)
Pathology
Antigens
CD34
Blood Pressure
Immunostaining
030204 cardiovascular system & hematology
Vascular Medicine
Biochemistry
Extracellular matrix
Tissue Culture Techniques
chemistry.chemical_compound
Adventitia
Arterial Pressure
Cells
Cultured
Humans
Hyaluronic Acid
Immunohistochemistry
Myocytes
Smooth Muscle
Saphenous Vein
Stem Cells
Tunica Intima
Tunica Media
Vasa Vasorum
Veins
Versicans
Biochemistry
Genetics and Molecular Biology (all)
Agricultural and Biological Sciences (all)
0302 clinical medicine
Animal Cells
Smooth Muscle
Hyaluronic acid
Medicine and Health Sciences
Adventitia/metabolism
Antigens
CD34/metabolism
Arterial Pressure/physiology
Hyaluronic Acid/metabolism
Myocytes
Smooth Muscle/metabolism
Saphenous Vein/cytology
Saphenous Vein/metabolism
Stem Cells/metabolism
Tunica Intima/cytology
Tunica Intima/metabolism
Tunica Media/cytology
Tunica Media/metabolism
Vasa Vasorum/cytology
Vasa Vasorum/metabolism
Veins/cytology
Veins/metabolism
Veins/transplantation
Versicans/genetics
Versicans/metabolism
Staining
Extracellular Matrix Proteins
Multidisciplinary
Cultured
biology
Chemistry
Physics
Cell Staining
Classical Mechanics
Tunica intima
medicine.anatomical_structure
Physical Sciences
cardiovascular system
Medicine
Versican
Anatomy
Cellular Types
Research Article
Tunica media
medicine.medical_specialty
Science
Cells
Research and Analysis Methods
03 medical and health sciences
Pressure
medicine
cardiovascular diseases
Antigens
Myocytes
Biology and Life Sciences
Proteins
Cell Biology
carbohydrates (lipids)
030104 developmental biology
Specimen Preparation and Treatment
Vasa vasorum
Cardiovascular Anatomy
biology.protein
Blood Vessels
CD34
Pericytes
Ex vivo
High Pressure
Zdroj: PloS one, vol. 13, no. 9, pp. e0204045
PLoS ONE
PLoS ONE, Vol 13, Iss 9, p e0204045 (2018)
Popis: Changes in extracellular matrix proteins may contribute significantly to the adaptation of vein grafts to the arterial circulation. We examined the production and distribution of versican and hyaluronan in intact human vein rings cultured ex vivo, veins perfused ex vivo, and cultured venous adventitial and smooth muscle cells. Immunohistochemistry revealed higher levels of versican in the intima/media compared to the adventitia, and no differences in hyaluronan. In the vasa vasorum, versican and hyaluronan associated with CD34+ progenitor cells. Culturing the vein rings for 14 days revealed increased versican immunostaining of 30-40% in all layers, with no changes in hyaluronan. Changes in versican accumulation appear to result from increased synthesis in the intima/media and decreased degradation in the adventitia as versican transcripts were increased in the intima/media, but unchanged in the adventitia, and versikine (the ADAMTS-mediated cleavage product of versican) was increased in the intima/media, but decreased in the adventitia. In perfused human veins, versican was specifically increased in the intima/media in the presence of venous pressure, but not with arterial pressure. Unexpectedly, cultured adventitial cells express and accumulate more versican and hyaluronan than smooth muscle cells. These data demonstrate a differential regulation of versican and hyaluronan in human venous adventitia vs. intima/media and suggest distinct functions for these extracellular matrix macromolecules in these venous wall compartments during the adaptive response of vein grafts to the arterial circulation.
Databáze: OpenAIRE
Externí odkaz: