Transcription factor KLF7 regulates differentiation of neuroectodermal and mesodermal cell lineages
| Autor: | Massimiliano Caiazzo, Francesco Ramirez, Umberto di Porzio, Silvia Parisi, Luca Colucci-D'Amato, Stefano Stifani, Maria Teresa Esposito |
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| Přispěvatelé: | Caiazzo, Massimiliano, Luca Colucci, D'Amato, Maria T., Esposito, Parisi, Silvia, Stefano, Stifani, Francesco, Ramirez, Umberto di, Porzio, Caiazzo, M, COLUCCI D'AMATO, Generoso Luca, Esposito, Mt, Parisi, S, Stifani, S, Ramirez, F, DI PORZIO, U. |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Cellular differentiation
Blotting Western Kruppel-Like Transcription Factors BLBP/FABP7 Map2 Biology PC12 Cells Mesoderm Small hairpin RNA Mice Animals Gene silencing Gene Silencing Cardiomyogenesi Neuritogenesi Neuritogenesis Transcription factor Cells Cultured Embryonic Stem Cells Homeodomain Proteins Mice Knockout Neurons Regulation of gene expression Neural Plate Adipogenesi Adipogenesis Reverse Transcriptase Polymerase Chain Reaction Osteogenesi Nanog Homeobox Protein Cell Differentiation Cell Biology Immunohistochemistry Neural stem cell Rats Gene Expression Regulation Cancer research Female Stem cell Cardiomyogenesis Octamer Transcription Factor-3 |
| Zdroj: | Experimental cell research 316 (2010): 2365–2376. info:cnr-pdr/source/autori:Caiazzo M, Colucci-D'Amato L, Esposito MT, Parisi S, Stifani S, Ramirez F, di Porzio U./titolo:Transcription factor KLF7 regulates differentiation of neuroectodermal and mesodermal cell lineages./doi:/rivista:Experimental cell research/anno:2010/pagina_da:2365/pagina_a:2376/intervallo_pagine:2365–2376/volume:316 |
| Popis: | Previous gene targeting studies in mice have implicated the nuclear protein Krüppel-like factor 7 (KLF7) in nervous system development while cell culture assays have documented its involvement in cell cycle regulation. By employing short hairpin RNA (shRNA)-mediated gene silencing, here we demonstrate that murine Klf7 gene expression is required for in vitro differentiation of neuroectodermal and mesodermal cells. Specifically, we show a correlation of Klf7 silencing with down-regulation of the neuronal marker microtubule-associated protein 2 (Map2) and the nerve growth factor (NGF) tyrosine kinase receptor A (TrkA) using the PC12 neuronal cell line. Similarly, KLF7 inactivation in Klf7-null mice decreases the expression of the neurogenic marker brain lipid-binding protein/fatty acid-binding protein 7 (BLBP/FABP7) in neural stem cells (NSCs). We also report that Klf7 silencing is detrimental to neuronal and cardiomyocytic differentiation of embryonic stem cells (ESCs), in addition to altering the adipogenic and osteogenic potential of mouse embryonic fibroblasts (MEFs). Finally, our results suggest that genes that are key for self-renewal of undifferentiated ESCs repress Klf7 expression in ESCs. Together with previous findings, these results provide evidence that KLF7 has a broad spectrum of regulatory functions, which reflect the discrete cellular and molecular contexts in which this transcription factor operates. © 2010 Elsevier Inc. |
| Databáze: | OpenAIRE |
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