Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes
| Autor: | Frank Sundler, Hindrik Mulder, Cecilia Holm, Anna Maria Hoffmann, Anders Bergh, Lena Stenson Holst, Gudrun Fredrikson |
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| Rok vydání: | 1994 |
| Předmět: |
Male
endocrine system Biophysics Hormone-sensitive lipase In situ hybridization Steroid biosynthesis Biochemistry Rats Sprague-Dawley Cholesterol ester hydrolase Structural Biology Pregnancy Lipid droplet Cryptorchidism Testis Genetics medicine Animals Lipase Molecular Biology In Situ Hybridization Mesylates Messenger RNA Sertoli Cells biology urogenital system Leydig Cells Cell Biology Sterol Esterase Sertoli cell Lipid Metabolism Molecular biology Rats Hormone sensitive lipase medicine.anatomical_structure biology.protein Female Spermatogenesis |
| Zdroj: | FEBS letters. 355(2) |
| ISSN: | 0014-5793 |
| Popis: | Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression. |
| Databáze: | OpenAIRE |
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