IL-7 Induces Myelopoiesis and Erythropoiesis
Autor: | Francesca B. Aiello, Glenn Dranoff, Renata Mazzucchelli, Scott K. Durum, Jonathan R. Keller, Kimberly D. Klarmann |
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Rok vydání: | 2007 |
Předmět: |
Myeloid
Immunology Stem cell factor Biology Interferon-gamma Mice medicine Animals Immunology and Allergy Erythropoiesis Progenitor cell Erythroid Precursor Cells Myelopoiesis Blood Cells Interleukin-7 Granulocyte-Macrophage Colony-Stimulating Factor Membrane Proteins Cell biology Haematopoiesis medicine.anatomical_structure Erythropoietin Cytokines Interleukin-3 Bone marrow Spleen medicine.drug |
Zdroj: | The Journal of Immunology. 178:1553-1563 |
ISSN: | 1550-6606 0022-1767 |
Popis: | IL-7 administration to mice was previously reported to increase the mobilization of progenitor cells from marrow to peripheral sites. We now report that IL-7 increases the number of mature myeloid and monocytic cells in spleen and peripheral blood. This effect required T cells, and we show that IL-7 treatment in vivo induced GM-CSF and IL-3 production by T cells with memory phenotype. However, additional myelopoietic cytokines were shown to be involved because mice deficient in both GM-CSF and IL-3 also responded to IL-7 with increased myelopoiesis. Candidate cytokines included IFN-γ and Flt3 ligand, which were also produced in response to IL-7. Because IFN-γ-deficient mice also increased myelopoiesis, it was suggested that IL-7 induced production of redundant myelopoietic cytokines. In support of this hypothesis, we found that the supernatant from IL-7-treated, purified T cells contained myelopoietic activity that required a combination of Abs against GM-CSF, IL-3, and anti-Flt3 ligand to achieve maximum neutralization. IL-7 administration increased the number of splenic erythroid cells in either normal, Rag1 or GM-CSF-IL-3-deficient mice, suggesting that IL-7 might directly act on erythroid progenitors. In support of this theory, we detected a percentage of TER-119+ erythroid cells that expressed the IL-7Rα-chain and common γ-chain. Bone marrow cells expressing IL-7R and B220 generated erythroid colonies in vitro in response to IL-7, erythropoietin, and stem cell factor. This study demonstrates that IL-7 can promote nonlymphoid hemopoiesis and production of cytokines active in the host defense system in vivo, supporting its possible clinical utility. |
Databáze: | OpenAIRE |
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