Performance of the currently available DIVA real-time PCR assays in classical and recombinant lumpy skin disease viruses
| Autor: | Irina Shumilova, Aleksandr Kononov, Svetlana Kononova, A.V. Sprygin, Yana Pestova, Alexander S. Nesterov, Olga Byadovskaya, Shawn Babiuk |
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| Rok vydání: | 2020 |
| Předmět: |
General Veterinary
General Immunology and Microbiology Lumpy Skin Disease Pcr assay Vaccine virus Cattle Diseases Viral Vaccines General Medicine Biology medicine.disease Real-Time Polymerase Chain Reaction Vaccines Attenuated Lumpy skin disease virus Virology law.invention Diva Real-time polymerase chain reaction law Lumpy skin disease Recombinant DNA medicine Animals Cattle Gene |
| Zdroj: | Transboundary and emerging diseasesREFERENCES. 68(6) |
| ISSN: | 1865-1682 |
| Popis: | The use of live homologous vaccines to protect against lumpy skin disease virus (LSDV) infection requires the use of molecular tools to differentiate between infected and vaccinated animals (DIVA). In this study, the commercial real-time PCR assays; ID Gene™ LSD DIVA Triplex kit and Bio-T kit® LSD - DIVA, as well as published assays targeting the GPCR gene (Journal of Virological Methods, 249, 48-57) and ORF008 and ORF126 (Sel'skokhozyaistvennaya Biologiya, 54, 347-358) were evaluated. These assays correctly identified classical field isolates (European lineage) and vaccine (Neethling vaccine). In contrast, when tested using vaccine-like recombinant viruses, the commercial and published assays were not able to correctly identify recombinant isolates. At the same time, the recombinant viruses were detected as either field and/or vaccine, or not detected at all depending on the assay. The different gene sequences present in recombinant viruses cause these DIVA assays to incorrectly assign recombinant viruses as either a field or vaccine virus. This observation has implications for using these assays and for identification of LSDV vaccine. |
| Databáze: | OpenAIRE |
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