P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation

Autor:	Marco Bocola, Matthias Wilmanns, Ulrich Schwaneberg, Marina Linow, Jochen Mueller-Dieckmann, Saravanan Panneerselvam, Aamir Shehzad, Danilo Roccatano
Rok vydání:	2013
Předmět:	Stereochemistry Stereoisomerism Crystal structure Crystallography X-Ray Catalysis Substrate Specificity Styrene chemistry.chemical_compound Residue (chemistry) Protein structure Molecular level Bacterial Proteins Cytochrome P-450 Enzyme System Oxidoreductase Materials Chemistry NADPH-Ferrihemoprotein Reductase chemistry.chemical_classification Binding Sites Metals and Alloys Enantioselective synthesis General Chemistry Protein Structure Tertiary Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Ceramics and Composites Epoxy Compounds
Zdroj:	Chemical Communications. 49:4694
ISSN:	1364-548X 1359-7345
DOI:	10.1039/c3cc39076d
Popis:	Solved crystal structures of P450 BM3 variants in complex with styrene provide on the molecular level a first explanation of how a positively charged surface residue inverts the enantiopreference of styrene epoxidation. The obtained insights into productive and non-productive styrene binding modes deepened our understanding of enantioselective epoxidation with P450 BM3.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::182631ab8cd391423d097f5c0a93b66b https://doi.org/10.1039/c3cc39076d Zobrazit plný text záznamu