Tyrosinase Inhibition Antioxidant Effect and Cytotoxicity Studies of the Extracts of Cudrania tricuspidata Fruit Standardized in Chlorogenic Acid

Autor: Dae-Hun Park, Sung-Ho Lee, In-Soo Yoon, Jung-Hyun Shim, Goo Yoon, Deuk-Sil Oh, Ha-Na Oh, Eunok Im, Seung Sik Cho, Hong-Seop Moon, Seung-Yub Song, Ji-Yeon Park, Sang Hoon Rhee
Rok vydání: 2019
Předmět:
Antioxidant
Cell Survival
medicine.medical_treatment
Tyrosinase
Pharmaceutical Science
C.tricuspidata Bureau
tyrosinase
High-performance liquid chromatography
Moraceae
Antioxidants
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
0404 agricultural biotechnology
Chlorogenic acid
lcsh:Organic chemistry
Phenols
In vivo
Cell Line
Tumor
Drug Discovery
medicine
Humans
Physical and Theoretical Chemistry
Enzyme Inhibitors
Cytotoxicity
Chromatography
High Pressure Liquid
030304 developmental biology
Flavonoids
0303 health sciences
Ethanol
Traditional medicine
Chemistry
Monophenol Monooxygenase
Plant Extracts
Communication
Organic Chemistry
food and beverages
Biological activity
04 agricultural and veterinary sciences
040401 food science
Chemistry (miscellaneous)
Fruit
Molecular Medicine
Chlorogenic Acid
HPLC
Zdroj: Molecules
Molecules, Vol 24, Iss 18, p 3266 (2019)
ISSN: 1420-3049
Popis: In the present study, various extracts of C. tricuspidata fruit were prepared with varying ethanol contents and evaluated for their biomarker and biological properties. The 80% ethanolic extract showed the best tyrosinase inhibitory activity, while the 100% ethanolic extract showed the best total phenolics and flavonoids contents. The HPLC method was applied to analyze the chlorogenic acid in C. tricuspidata fruit extracts. The results suggest that the observed antioxidant and tyrosinase inhibitory activity of C. tricuspidata fruit extract could partially be attributed to the presence of marker compounds in the extract. In this study, we present an analytical method for standardization and optimization of C. tricuspidata fruit preparations. Further investigations are warranted to confirm the in vivo pharmacological activity of C. tricuspidata fruit extract and its active constituents and assess the safe use of the plant for the potential development of the extract as a skin depigmentation agent.
Databáze: OpenAIRE
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