The multi-ligand somatostatin analogue SOM230 inhibits ACTH secretion by cultured human corticotroph adenomas via somatostatin receptor type 5
| Autor: | Diana Sprij-Mooij, Joost van der Hoek, Marlijn Waaijers, Leo J. Hofland, Steven W. J. Lamberts, Maarten O. van Aken, Wouter W. de Herder, Gisbert Weckbecker, Peter M. van Koetsveld, Christian Bruns, Richard A Feelders, Albert Beckers |
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| Přispěvatelé: | Internal Medicine |
| Rok vydání: | 2005 |
| Předmět: |
Adenoma
endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism Gene Expression Adrenocorticotropic hormone Somatostatin Receptor Type 5 Biology Pituitary neoplasm Octreotide chemistry.chemical_compound Mice Endocrinology Adrenocorticotropic Hormone Internal medicine medicine Tumor Cells Cultured Animals Humans Pituitary Neoplasms RNA Messenger Receptors Somatostatin Receptor Glucocorticoids Reverse Transcriptase Polymerase Chain Reaction General Medicine medicine.disease Pasireotide Somatostatin chemistry Corticotropic cell hormones hormone substitutes and hormone antagonists |
| Zdroj: | European Journal of Endocrinology, 152(4), 645-654. Bioscientifica Ltd |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/eje.1.01876 |
| Popis: | Objective: Currently, there is no effective medical treatment for patients with pituitary-dependent Cushing’s disease. A novel somatostatin (SS) analogue, named SOM230, with high binding affinity to SS receptor subtypes sst1, sst2, sst3 and sst5 was recently introduced. We compared the in vitro effects of the sst2-preferring SS analogue octreotide (OCT) and the multi-ligand SOM230 on ACTH release by human and mouse corticotroph tumour cells. Methods: By quantitative RT-PCR the sst subtype expression level was determined in human corticotroph adenomas. In vitro, the inhibitory effect of OCT and SOM230 on ACTH release by dispersed human corticotroph adenoma cells and mouse AtT20 corticotroph adenoma cells was determined. In addition, the influence of dexamethasone on the responsiveness to OCT and SOM230 was studied. Results: Corticotroph adenomas expressed predominantly sst5 mRNA (six out of six adenomas), whereas sst2 mRNA expression was detected at significantly lower levels. In a 72 h incubation with 10 nmol/l SOM230, ACTH release was inhibited in three out of five cultures (range −30 to −40%). Ten nmol/l OCT slightly inhibited ACTH release in only one of five cultures (− 28%). In AtT20 cells, expressing sst2, sst3 and sst5, SOM230 inhibited ACTH secretion with high potency (IC50 0.2 nmol/l). Dexamethasone (10 nmol/l) pre-treatment did not influence the sensitivity of the cells to the inhibitory effect of SOM230, suggesting that sst5 is relatively resistant to negative control by glucocorticoids. Conclusions: The selective expression of sst5 receptors in corticotroph adenomas and the preferential inhibition of ACTH release by human corticotroph adenoma cells by SOM230 in vitro, suggest that SOM230 may have potential in the treatment of patients with pituitary-dependent Cushing’s disease. |
| Databáze: | OpenAIRE |
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