Coagulation activity of circulating membrane microparticles in patients with cardiovascular diseases

Autor: O A, Antonova, N V, Golubeva, V V, Yakushkin, I T, Zyuryaev, E N, Krivosheeva, A L, Komarov, T V, Martynyuk, A V, Mazurov
Rok vydání: 2022
Předmět:
Zdroj: Biomeditsinskaya Khimiya. 68:288-296
ISSN: 2310-6905
2310-6972
DOI: 10.18097/pbmc20226804288
Popis: Membrane microparticles (MP) are released by activated or damaged cells and are able to accelerate blood clotting (coagulation). MP possess coagulation activity since all of them contain on their surface phosphatidylserine (PS), a substrate for the assembly of coagulation complexes, and some of them tissue factor (TF), the primary initiator of coagulation cascade reactions. We compared the coagulation activity and amount of MP in the blood of healthy donors (n=34) and patients with myocardial infarction (MI) (n=32), advanced atherosclerosis (AA) (n=32) and idiopathic pulmonary arterial hypertension (IPAH) (n=19). Total MP fraction was obtained from blood plasma by sedimentation at 20000 g, 30 min. The coagulation activity of PM isolated from 100 μl of donor and patient plasma was determined using a modified recalcification test. MP were added to substrate plasma devoid of endogenous MF, plasma was recalcified, and clotting was recorded by changes in optical density (A450), determining lag phase (min) and maximum rate (Vmax, %A450/min). MP were counted by flow cytometry as PS+ particles (lactadgerin-FITC staining) smaller than 1 μm and their concentration was expressed as 105 MP/μl plasma. MP in all patient groups accelerated plasma clotting more effectively than donor MP. Lag phase compared with donors (11.8 [11.0-13.1] median and interquartile range) was shorter in patients with AA (8.8 [7.0-10.3], p.Membrannye mikrochastitsy (MCh) vysvobozhdaiutsia aktivirovannymi ili povrezhdennymi kletkami i obladaiut sposobnost'iu uskoriat' svertyvanie (koaguliatsiiu) krovi. Koaguliatsionnaia aktivnost' MCh opredeliaetsia tem, chto vse oni soderzhat na svoeĭ poverkhnosti fosfatidilserin (FS), kotoryĭ sluzhit substratom dlia sborki koaguliatsionnykh kompleksov, a nekotorye iz nikh tkanevoĭ faktor (TF) — pervichnyĭ initsiator reaktsiĭ koaguliatsionnogo kaskada. My sravnivali koaguliatsionnuiu aktivnost' i kolichestvo MCh v krovi zdorovykh donorov (n=34) i patsientov s infarktom miokarda (IM) (n=32), rasprostranennym aterosklerozom (RA) (n=32) i idiopaticheskoĭ legochnoĭ arterial'noĭ gipertenzieĭ (ILAG) (n=19). Obshchuiu fraktsiiu MCh iz plazmy krovi poluchali osazhdeniem pri 20000 g, 30 min. Kontsentratsiiu MCh i stepen' ékspressii na ikh poverkhnosti FS opredeliali metodom protochnoĭ tsitometrii. Koaguliatsionnuiu aktivnost' MCh otsenivali s pomoshch'iu modifitsirovannogo testa rekal'tsifikatsii plazmy. Kontsentratsiia MCh po sravneniiu so zdorovymi donorami byla vyshe u patsientov s RA, nizhe u patsientov s IM i dostoverno ne otlichalas' u patsientov s ILAG. MCh vo vsekh gruppakh patsientov uskoriali svertyvanie plazmy éffektivnee, chem MCh zdorovykh donorov. Lag-faza u patsientov s RA, IM i ILAG byla koroche po sravneniiu so zdorovymi donorami, a Vmaks v sravnenii s zdorovymi donorami byla vyshe u patsientov s RA i ILAG, no ne IM. Vo vsekh gruppakh patsientov otmechena dostovernaia korreliatsiia mezhdu kontsentratsieĭ MCh i parametrami 1/lag-faza i Vmaks svertyvaniia plazmy. Poluchennye dannye pokazyvaiut, chto uvelichenie obshcheĭ koaguliatsionnoĭ aktivnosti MCh mozhet byt' ob"iasneno povysheniem ikh kontsentratsii tol'ko u patsientov s IM. Poskol'ku anti-TF antitela ne izmeniali parametry svertyvaniia v prisutstvii MCh kak zdorovykh donorov, tak i patsientov, TF, ochevidno, ne uchastvuet v realizatsii koaguliatsionnykh svoĭstv testiruemykh MCh. V to zhe vremia vo vsekh gruppakh patsientov nabliudali dostovernoe uvelichenie ékspressii FS na poverkhnosti MCh po sravneniiu so zdorovymi donorami, chto mozhet byt' odnoĭ iz prichin ikh povyshennoĭ koaguliatsionnoĭ aktivnosti.
Databáze: OpenAIRE
Externí odkaz: