LncRNA DLX6-AS1/miR-129-5p/DLK1 axis aggravates stemness of osteosarcoma through Wnt signaling
| Autor: | Hai-Ming Yu, Xue-Dong Yao, Rong-mou Zhang, Ting Tang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
musculoskeletal diseases 0301 basic medicine medicine.medical_treatment Biophysics Mice Nude Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Cancer stem cell Cell Line Tumor medicine Animals Humans Wnt Signaling Pathway neoplasms Molecular Biology Mice Inbred BALB C Osteosarcoma Chemotherapy Calcium-Binding Proteins Wnt signaling pathway Membrane Proteins Cell Biology DLX6 Prognosis medicine.disease Survival Analysis Up-Regulation Gene Expression Regulation Neoplastic Radiation therapy MicroRNAs 030104 developmental biology DLK1 Primary bone 030220 oncology & carcinogenesis Neoplastic Stem Cells Cancer research Intercellular Signaling Peptides and Proteins RNA Long Noncoding Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 507:260-266 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2018.11.019 |
| Popis: | Osteosarcoma is the most common primary bone tumor and occurs most frequently in adolescents. Cancer stem cells (CSCs) are resistant to chemotherapy and radiotherapy, and can drive cancer recurrence. In this study, we aimed to investigate the effect of Long noncoding RNAs (lncRNAs) DLX6-AS1 on osteosarcoma stemness and the underlying mechanism involved. DLX6-AS1 enhanced osteosarcoma stemness in vitro and in vivo. Moreover, DLX6-AS1 competitively interacted with miR-129-5p to DLK1, resulting in activation of Wnt signaling and promotion of stemness in osteosarcoma. DLX6-AS1 functionally interplayed with miR-129-5p to form a reciprocal feedback loop to activate Wnt signaling. High DLX6-AS1 expression was observed in osteosarcoma tissues, and predicted a poor prognosis for osteosarcoma patients. Our study suggests that DLX6-AS1, combined with miR-129-5p and DLK1, can be utilized as factors for the clinical diagnosis and prognosis of osteosarcoma, and may be potential targets for the treatment of osteosarcoma. |
| Databáze: | OpenAIRE |
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