Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni
| Autor: | Chrystelle Lasica, Hélène Moné, Cristian Chaparro, Christoph Grunau, Agnieszka Zelisko-Schmidt, Ronaldo de Carvalho Augusto, Gabriel Mouahid, Anne-Clémence Veillard |
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| Přispěvatelé: | Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), diagenode, Modat, Anne, Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
0303 health sciences
limited biological material biology Human blood library input 030231 tropical medicine ChIPmentation Medicine (miscellaneous) Computational biology Schistosoma mansoni biology.organism_classification Biological materials General Biochemistry Genetics and Molecular Biology ChIP-seq 03 medical and health sciences 0302 clinical medicine Human parasite [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Chromatin immunoprecipitation [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology 030304 developmental biology N-ChIP |
| Zdroj: | Wellcome Open Research Wellcome Open Research, 2022, 7, pp.133. ⟨10.12688/wellcomeopenres.17779.1⟩ |
| ISSN: | 2398-502X |
| Popis: | In living cells, the genetic information stored in the DNA sequence is always associated with chromosomal and extra-chromosomal epigenetic information. Chromatin is formed by the DNA and associated proteins, in particular histones. Covalent histone modifications are important bearers of epigenetic information and as such have been increasingly studied since about the year 2000. One of the principal techniques to gather information about the association between DNA and modified histones is chromatin immunoprecipitation (ChIP), also combined with massive sequencing (ChIP-Seq). Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq in many model species, using in general roughly 106 cells per experiment. Such high cell numbers are sometimes difficult to produce. Using the human parasite Schistosoma mansoni, whose production requires sacrificing animals and should therefore be kept to a minimum, we show here that automated ChIPmentation is suitable for limited biological material. We define the operational limit as ≥20,000 Schistosoma cells. We also present a streamlined protocol for the preparation of ChIP input libraries. |
| Databáze: | OpenAIRE |
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