Application of Four Anti-Human Interferon-α Monoclonal Antibodies for Immunoassay and Comparative Analysis of Natural Interferon-α Mixtures
| Autor: | Erik Lundgren, Hans-Peter Ekre, Gudrun Andersson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.drug_class
Immunoblotting Immunology Alpha interferon Enzyme-Linked Immunosorbent Assay Biology Monoclonal antibody law.invention Iodine Radioisotopes law Virology medicine Humans Interferon alfa medicine.diagnostic_test Isoelectric focusing Antibodies Monoclonal Molecular biology Recombinant Proteins Solutions Biochemistry Polyclonal antibodies Immunoassay Interferon Type I biology.protein Recombinant DNA Biological Assay Adsorption Isoelectric Focusing Antibody medicine.drug |
| Zdroj: | Journal of Interferon Research. 11:53-60 |
| ISSN: | 0197-8357 |
| DOI: | 10.1089/jir.1991.11.53 |
| Popis: | Four different mouse monoclonal antibodies to human interferon-alpha (IFN-alpha) were evaluated for application in quantitative and comparative analysis of natural IFN-alpha mixtures. Binding to IFN-alpha subtypes in solution revealed individual reactivity patterns. These patterns changed if the IFN-alpha molecules were immobilized either passively to a surface or bound by another antibody. Also, substitution of a single amino acid in IFN-alpha 2 affected the binding, apparently by altering the conformation. Isoelectric focusing of three natural IFN-alpha preparations from different sources, followed by immunoblotting, resulted in individual patterns with each of the four mAbs and also demonstrated variation in the composition of the IFN-alpha preparations. None of the mAbs was subtype specific, but by combining the different mAbs, and also applying polyclonal anti-human IFN-alpha antibodies, it was possible to design sensitive sandwich ELISAs with broad or more limited IFN-alpha subtype specificity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|