Molecular genotyping of methicillin-resistant Staphylococcus aureus strains in a teaching hospital in Turkey
| Autor: | A. Yasemin Öztop, M. Zahir Bakici, Hatice Pinarbasi, Mehmet Bakir, Sesin Kocagöz |
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| Přispěvatelé: | Cumhuriyet Univ, Fac Med, Dept Microbiol, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Fac Med, Dept Clin Microbiol, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Fac Med, Dept Biochem, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Fac Med, Dept Infect Dis & Clin Bacteriol, TR-58140 Sivas, Turkey -- Sabanci Univ, Fac Engn & Nat Sci, Istanbul, Turkey |
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Staphylococcus aureus Genotype Turkey Tetracycline Immunology Microbial Sensitivity Tests medicine.disease_cause Microbiology Polymerase Chain Reaction law.invention SmaI law Drug Resistance Bacterial medicine Pulsed-field gel electrophoresis Humans Hospitals Teaching Polymerase chain reaction DNA Primers Pharmacology Cross Infection business.industry biochemical phenomena metabolism and nutrition Staphylococcal Infections bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Virology Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Penicillin Gentamicin Methicillin Resistance business medicine.drug |
| Zdroj: | Microbial drug resistance (Larchmont, N.Y.). 10(2) |
| ISSN: | 1076-6294 |
| Popis: | WOS: 000222491200011 PubMed ID: 15256031 Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of nosocomial infections in our hospital. Therefore, we aimed to characterize MRSA isolates phenotypically from patients with nosocomial infections at Cumhuriyet University Hospital between December, 1999, and June, 2001, in Sivas by analysis of antibiotic patterns and genotypically using pulsed-field gel electrophoresis (PFGE) and repetitive element sequence-based polymerase chain reaction (rep-PCR). Forty-three nosocomial isolates were collected from various wards. All isolates were resistant to penicillin, tetracycline, oxacillin, and gentamicin. By rep-PCR and by separation of SmaI fragments of genomic DNA using PFGE, one major type (eight subtypes with PFGE) was identified among the strains. This clone was found to be different than some clones such as Iberian, Brazilian, and a major clone that was found in another Turkish University Hospital in Ankara. According to our results, there is a major MRSA clone with a potential to spread in our hospital. Infection control measures should be directed toward restricting the further spread of this clone. Therefore, in accordance with these findings, a surveillance culturing program should be established. |
| Databáze: | OpenAIRE |
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