Occurrence and Nature of Off-Target Modifications by CRISPR-Cas Genome Editing in Plants
| Autor: | Mark H. J. Sturme, Jan Pieter van der Berg, Lianne M. S. Bouwman, Adinda De Schrijver, Ruud A. de Maagd, Gijs A. Kleter, Evy Battaglia-de Wilde |
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| Rok vydání: | 2022 |
| Předmět: |
Team Bacteriologie
Moleculaire Biologie & AMR Team Agrochains off-target modifications Team Bacteriology Molecular Biology & AMR plants Moleculaire Biologie & AMR fungi food and beverages Plant Science Molecular Biology & AMR Agricultural and Biological Sciences (miscellaneous) Team Bacteriology genome editing Team Bacteriologie BIOS Plant Development Systems EPS CRISPR-Cas Agronomy and Crop Science Food Science |
| Zdroj: | ACS Agricultural Science and Technology 2 (2022) 2 ACS Agricultural Science and Technology, 2(2), 192-201 |
| ISSN: | 2692-1952 |
| DOI: | 10.1021/acsagscitech.1c00270 |
| Popis: | CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of plants. Nevertheless, unintended off-target edits can arise that might confer risks when present in gene-edited food crops. Through an extensive literature review we gathered information on CRISPR-Cas off-target edits in plants. Most observed off-target changes were small insertions or deletions (1-22 bp) or nucleotide substitutions, and large deletions (>100 bp) were rare. One study detected the insertion of vector-derived DNA sequences, which is important considering the risk assessment of gene-edited plants. Off-target sites had few mismatches (1-3 nt) with the target sequence and were mainly located in protein-coding regions, often in target gene homologues. Off-targets edits were predominantly detected via biased analysis of predicted off-target sites instead of unbiased genome-wide analysis. CRISPR-Cas-edited plants showed lower off-target mutation frequencies than conventionally bred plants. This Review can aid discussions on the relevance of evaluating off-target modifications for risk assessment of CRISPR-Cas-edited plants. |
| Databáze: | OpenAIRE |
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