Effect of cariporide on ram sperm pH regulation and motility: possible role of NHE1
| Autor: | Maria Teresa Roscino, Elena Ciani, Ciro Leonardo Pierri, Lorenzo Guerra, Antonio Ciro Guaricci, Maria Favia, Nicola Antonio Martino, Giovanni Michele Lacalandra, Giuseppe Punzi, Maria Elena Dell'Aquila, Valeria Casavola, F Silvestre, Stefania Muzzachi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Embryology Intracellular pH Motility Guanidines 03 medical and health sciences chemistry.chemical_compound Endocrinology Capacitation Animals Sulfones Sperm motility Sheep Sodium-Hydrogen Exchanger 1 Cariporide Chemistry Obstetrics and Gynecology Cell Biology Basolateral plasma membrane Hydrogen-Ion Concentration Sperm Spermatozoa Cell biology Sodium–hydrogen antiporter 030104 developmental biology Reproductive Medicine Sperm Motility Sperm Capacitation |
| Zdroj: | Reproduction (Cambridge, England). 155(5) |
| ISSN: | 1741-7899 |
| Popis: | Sperm motility, a feature essential forin vivofertilization, is influenced by intracellular pH (pHi) homeostasis. Several mechanisms are involved in pHiregulation, among which sodium–hydrogen exchangers (NHEs), a family of integral transmembrane proteins that catalyze the exchange of Na+for H+across lipid bilayers. A preliminary characterization of NHE activity and kinetic parameters, followed by analysis of the expression and localization of the protein in ram spermatozoa was performed. NHE activity showed an apparentKmfor external Na+of 17.61 mM. Immunoblotting revealed a molecular mass of 85 kDa. Immunolocalization pattern showed some species-specific aspects, such as positive labeling at the equatorial region of the sperm head. Cariporide, a selective NHE1 inhibitor, significantly reduced pHirecovery (85%). Similarly, exposure to cariporide significantly inhibited different motility parameters, including those related to sperm capacitation.In vitrofertilization (IVF) was not affected by cariporide, possibly due to the non-dramatic, although significant, drop in motility and velocity parameters or due to prolonged exposure during IVF, which may have caused progressive loss of its inhibitory effect. In conclusion, this is the first study documenting, in a large animal model (sheep) of well-known translational relevance, a direct functional role of NHE on sperm pHiand motility. The postulated specificity of cariporide toward isoform 1 of the Na+/H+exchanger seems to suggest that NHE1 may contribute to the observed effects on sperm cell functionality. |
| Databáze: | OpenAIRE |
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