Comparison of the TIM and TOM channel activities of the mitochondrial protein import complexes
| Autor: | Kathleen W. Kinnally, Serguei M. Grigoriev, Dawn Pietkiewicz, Concepción Muro, Maria Luisa Campo |
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| Rok vydání: | 2003 |
| Předmět: |
Voltage-dependent anion channel
biology Translocase of the outer membrane Biophysics TIM/TOM complex Intracellular Membranes Protein Sorting Signals Mitochondrial Membrane Transport Proteins Ion Channels Cell biology Membrane Potentials Mitochondria Mitochondrial membrane transport protein Protein Transport Membrane Channels Receptors and Transporters Yeasts Translocase of the inner membrane biology.protein Bacterial outer membrane Ion channel |
| Zdroj: | Biophysical journal. 84(5) |
| ISSN: | 0006-3495 |
| Popis: | Water-filled channels are central to the process of translocating proteins since they provide aqueous pathways through the hydrophobic environment of membranes. The Tom and Tim complexes translocate precursors across the mitochondrial outer and inner membranes, respectively, and contain channels referred to as TOM and TIM (previously called PSC and MCC). In this study, little differences were revealed from a direct comparison of the single channel properties of the TOM and TIM channels of yeast mitochondria. As they perform similar functions in translocating proteins across membranes, it is not surprising that both channels are high conductance, voltage-dependent channels that are slightly cation selective. Reconstituted TIM and TOM channel activities are not modified by deletion of the outer membrane channel VDAC, but are similarly affected by signal sequence peptides. |
| Databáze: | OpenAIRE |
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