Effects of nitric oxide donor and nitric oxide synthase inhibitor on ruminal contractions in conscious sheep
Autor: | W. Korczynski, Takenori Onaga, Hiroyuki Okada, C. Nagashima, Hiroki Inoue, S. Hagiwara, Seiyu Kato |
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Rok vydání: | 2002 |
Předmět: |
Male
medicine.medical_specialty Rumen Arginine Endogeny S-Nitroso-N-Acetylpenicillamine Nitric oxide chemistry.chemical_compound Basal (phylogenetics) Micromol/kg Internal medicine medicine Pressure Animals Nitric Oxide Donors Enzyme Inhibitors Sheep General Veterinary biology Snap Muscle Smooth Nitric oxide synthase Kinetics Endocrinology NG-Nitroarginine Methyl Ester chemistry Anesthesia biology.protein Nitric Oxide Synthase Gastrointestinal Motility Muscle Contraction |
Zdroj: | Research in veterinary science. 71(3) |
ISSN: | 0034-5288 |
Popis: | The present study was planned to evaluate a role of nitric oxide (NO) in the regulation of regular ruminal contractions in conscious sheep. Intravenous infusion of S-nitroso-acetyl-DL-penicillamine (SNAP) at doses of 3-30 nmol kg(-1) min(-1)for 30 minutes inhibited both the amplitude and frequency of ruminal contractions in a dose-dependent manner. However, intravenous infusion of Nomega-nitro-L-arginine-methyl ester (L-NAME) at doses of 0.3-3.0 micromol kg(-1) min(-1)did not alter the basal tone of intraruminal pressure and the amplitude of ruminal contractions. The frequency of contractions was slightly inhibited by L-NAME infusion at 1.0 micromol kg(-1)min(-1). The effects of L-NAME were abolished by simultaneous infusion of L -arginine at 30 micromol kg(-1) min(-1). These results suggest that exogenous NO can diminish the ruminal contractions, while endogenous NO is not involved in the regulatory mechanism of basal tone and regular phasic contractions of the rumen in healthy sheep. |
Databáze: | OpenAIRE |
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