Intestinal schistosomiasis among preschool and school-aged children in a rural setting near Alexandria: initiative for elimination
| Autor: | Hoda Fahmy Farag, Amal Farahat Allam, Musaad Mohammed Dewair, Heba Said Ibrahim, Amel Youssef Shehab, Aziza Ibrahim Salem, Amel Elsheredy, Nancy Abd-elkader Hagras |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Rural Population medicine.medical_specialty Intestinal schistosomiasis Stool sample Melting temperature 030231 tropical medicine Real-Time Polymerase Chain Reaction Praziquantel 03 medical and health sciences fluids and secretions 0302 clinical medicine Schistosomiasis control Internal medicine parasitic diseases medicine Prevalence Animals Humans Child Anthelmintics School age child biology business.industry Public Health Environmental and Occupational Health Rural setting Infant Schistosoma mansoni biology.organism_classification Schistosomiasis mansoni Infectious Diseases Treatment Outcome Child Preschool Parasitology Egypt Female business medicine.drug |
| Zdroj: | Tropical medicineinternational health : TMIHReferences. 26(6) |
| ISSN: | 1365-3156 |
| Popis: | OBJECTIVE To assess the status of intestinal schistosomiasis among preschool-aged (PSAC) and school-aged children (SAC) and to compare the efficacy of praziquantel (PZQ) in both groups. METHODS The study was conducted on 400 children; 103 PSAC and 297 SAC. Diagnosis of Schistosoma mansoni was based on triplicate Kato-Katz thick smears from a single stool sample. To identify the missed cases by Kato-Katz, 120 randomly selected negative cases (38 PSAC and 82 SAC) were screened by real-time PCR. All S. mansoni-positive cases by Kato-Katz were treated by crushed PZQ tablets. Four weeks after treatment, the cure rate was assessed by Kato-Katz smears and real-time PCR. RESULTS The prevalence of S. mansoni with Kato-Katz was 7.8% among PSAC and 7.4% among SAC. Most of children (63.3%) had light-intensity infection. The cure rate was 100% among PSAC by both techniques, and 91%, and 77.2% among SAC by Kato-Katz and real-time PCR, respectively. In the 120 stool samples screened by real-time PCR, S. mansoni prevalence was 25%; 15.8% and 29.3% were among PSAC and SAC respectively. Treated cases showed a lower range of Ct values than untreated cases. Two melting temperature ranges (Tm = 83-87°C and 89-93°C) were recognised among uncured cases which may point to S. mansoni genetic variability. CONCLUSION Continuous monitoring and inclusion of PSAC in schistosomiasis control programmes are crucial. Real-time PCR and other molecular tools are recommended for evaluation of the true prevalence, assessment of cure and further studies on genetic diversity. |
| Databáze: | OpenAIRE |
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