Zinc and Copper in Milk and Tissues of Nursing Lethal Milk Mutant Mice
| Autor: | Harvey R. Herschman, Bo Lönnerdal, John E. Piletz, Wade L. Berry, Lucille S. Hurley, Roger E. Ganschow |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
Mammary gland Mutant Medicine (miscellaneous) chemistry.chemical_element Zinc Biology Mice Fetus Pregnancy Internal medicine Lactation medicine Animals Intestinal Mucosa Copper levels Micelles Nutrition and Dietetics Osmolar Concentration Glutamate receptor medicine.disease Copper Mice Mutant Strains Animals Suckling Molecular Weight Milk medicine.anatomical_structure Endocrinology Liver chemistry Zinc deficiency Female Metallothionein |
| Zdroj: | The Journal of Nutrition. 117:83-90 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/117.1.83 |
| Popis: | Zinc concentration was lower in liver of suckling 1-d-old lethal milk (lm/lm) mutant mice than in wild-type pups, in accordance with the hypothesis of milk-induced zinc deficiency previously proposed to underlie this mutation. Despite the initial deficiency, by 3 d of age suckling lm/lm pups exhibited higher levels of hepatic zinc than did lm/lm-nursed wild-type pups. Intestinal zinc and copper concentrations were normal in 1-d-old lm/lm pups, but by 3 d of age were also higher in lm/lm pups than in wild-type pups foster-nursed on lm/lm dams. Contrary to a previous report, we found that zinc concentration in milk of lm/lm dams was not significantly different from those of controls, between 4-20 d postpartum. Mutant milk showed normal zinc distribution as determined by gel-filtration chromatography or by DEAE-cellulose chromatography of zinc-binding ligands derived from EDTA-dissociated micelles, normal copper levels, normal amounts of citrate, a zinc (II) and copper (II)-binding ligand and normal amounts of glutamate, a proposed copper (II)-binding ligand. Total mammary glands and mammary gland cytosols from lm/lm mice exhibited normal zinc concentrations. Copper levels, however, were higher in lm/lm mammary gland cytosols than in controls. These results suggest that an increased uptake and/or retention of zinc and copper in the tissues studied may underlie the signs of zinc deficiency seen in lethal milk mutant mice. |
| Databáze: | OpenAIRE |
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