Human feeder cell line for derivation and culture of hESc/hiPSc
| Autor: | Maureen Mee, Banu Iskender, Tristan R. McKay, John D. Aplin, Daniel E. Foxler, Maria V. Camarasa, Duncan Miller, Jayne C. Fitzsimmons, Susan J. Kimber, Tyson V. Sharp, Nicola Bates, Jinpei Ye, Daniel R. Brison |
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| Rok vydání: | 2011 |
| Předmět: |
Pluripotent Stem Cells
Cell Culture Techniques Biology Cell Line Second trimester In Situ Nick-End Labeling Humans Telomerase reverse transcriptase Stromal fibroblasts Induced pluripotent stem cell Embryonic Stem Cells Cell Proliferation Medicine(all) Cell Differentiation General Medicine Cell Biology Flow Cytometry Embryonic stem cell Immunohistochemistry Cell biology Feeder Cell Cell culture embryonic structures Line (text file) biological phenomena cell phenomena and immunity Developmental Biology |
| Zdroj: | Stem Cell Research. 7(2):154-162 |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2011.04.005 |
| Popis: | We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use. (C) 2011 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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