The RNA-binding protein ATX-2 regulates cytokinesis through PAR-5 and ZEN-4
| Autor: | Masaki Shirayama, Eddie G. Dominguez, Alex R. Villarreal, Ahna R. Skop, Megan M. Gnazzo, Eva-Maria E. Uhlemann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Kinesins Mitosis Spindle Apparatus Biology Microtubules 03 medical and health sciences 0302 clinical medicine Microtubule Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Ataxin-2 Cytokinesis Centrosome fungi Spindle midzone Cell Cycle RNA-Binding Proteins Cell Biology Articles Cell biology Spindle apparatus Midbody 030104 developmental biology RNA Microtubule-Associated Proteins 030217 neurology & neurosurgery Centrosome localization |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | The mechanisms that mediate the temporal and spatial recruitment of cell division factors to the spindle midzone and midbody remain unclear. Cell division is regulated by the conserved RNA-binding protein, ATX-2/Ataxin-2, which facilitates the targeting of ZEN-4 to the spindle midzone by mediating PAR-5. The spindle midzone harbors both microtubules and proteins necessary for furrow formation and the completion of cytokinesis. However, the mechanisms that mediate the temporal and spatial recruitment of cell division factors to the spindle midzone and midbody remain unclear. Here we describe a mechanism governed by the conserved RNA-binding protein ATX-2/Ataxin-2, which targets and maintains ZEN-4 at the spindle midzone. ATX-2 does this by regulating the amount of PAR-5 at mitotic structures, particularly the spindle, centrosomes, and midbody. Preventing ATX-2 function leads to elevated levels of PAR-5, enhanced chromatin and centrosome localization of PAR-5–GFP, and ultimately a reduction of ZEN-4–GFP at the spindle midzone. Codepletion of ATX-2 and PAR-5 rescued the localization of ZEN-4 at the spindle midzone, indicating that ATX-2 mediates the localization of ZEN-4 upstream of PAR-5. We provide the first direct evidence that ATX-2 is necessary for cytokinesis and suggest a model in which ATX-2 facilitates the targeting of ZEN-4 to the spindle midzone by mediating the posttranscriptional regulation of PAR-5. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|