Extract, fractions, and ethyl-p-methoxycinnamate isolate from Kaempferia galanga Elicit anti-inflammatory activity by limiting leukotriene B4 (LTB4) production

Autor: Supandi, Ni Putu Ermi Hikmawanti, Yeni, Lusi Putri Dwita
Rok vydání: 2021
Předmět:
HF
n-hexane fraction
LTB4
leukotriene B4
Leukotriene B4
medicine.drug_class
Ginger Extract
Lipoxygenase
AA
arachidonic acid
TBA
thiobarbituric acid
Granuloma-air pouch
EPMC
ethyl-p-methoxycinnamate
TNF-α
tumor necrosis factor-α
Article
Anti-inflammatory
chemistry.chemical_compound
EAF
ethyl acetate fraction
Anti-inflammation
medicine
Kaempferia galanga
PG
prostaglandin
E
ethanol extract
Pleurisy
LOX
lipoxygenase
FLAP
5-lipoxygenase-activating protein
ARDS
acute respiratory distress syndrome
WF
water fraction
MDA
malondialdehyde
LTA4H
LTA4 hydrolase
IBD
inflammatory bowel disease
Traditional medicine
biology
Zileuton
TEP
tetraethoxypropane
biology.organism_classification
Malondialdehyde
IL
interleukin
Carrageenan
COX
cyclooxygenase
COPD
chronic obstructive pulmonary disease
Complementary and alternative medicine
chemistry
biology.protein
Medicine
medicine.drug
Zdroj: Journal of Traditional and Complementary Medicine, Vol 11, Iss 6, Pp 563-569 (2021)
Journal of Traditional and Complementary Medicine
ISSN: 2225-4110
DOI: 10.1016/j.jtcme.2021.06.004
Popis: Background and aim Kaempferia galanga, also known as aromatic Ginger (kencur) in Indonesia, has been widely explored and shows potential as an anti-inflammatory agent. However, there has been limited research to show a possible mechanism by which aromatic ginger inhibits lipoxygenase (LOX). Therefore, this study aims to determine the anti-inflammatory activity of aromatic ginger by comparing extract, fractions, and ethyl-p-methoxycinnamate (EPMC) isolate, as well as possible LOX inhibition activity, by reducing the production of leukotriene B4 (LTB4). Experimental procedure Two animal models were used, namely, the carrageenan-induced granuloma air pouch model and the pleurisy model. The test substance was administered 1 h before carrageenan induction, which was performed orally for each animal model. The number of leukocytes and the malondialdehyde (MDA) levels, leukotriene B4 (LTB4) levels, and histology were observed. GC-MS and LC-MS were used for analysis of the chemical compounds in the test samples. Results and conclusion The results of GC-MS analysis showed that aromatic ginger rhizome extract and fractions were dominated by ethyl-trans-p-methoxycinnamate, with the highest level found in the extract. K. galanga showed significant anti-inflammatory activity compared to the control (p
Graphical abstract Image 1
Highlights • Comparison of extract, fraction and isolate of Kamperia galanga anti-inflammation activity. • Anti-inflammatory activity of Kaempferia galanga in pleurisy model. • Extract, fraction and isolate of Kamperia galanga as LOX inhibitor.
Databáze: OpenAIRE
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