Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen
| Autor: | Tan Liu, Jing Wang, Qing Wei, Shuliang Jiao, Hao Zhang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Capillary action Measure (physics) chemistry.chemical_element 02 engineering and technology 01 natural sciences Oxygen Multimodal Imaging Imaging phantom 010309 optics Optical coherence tomography 0103 physical sciences Microscopy medicine ocis:(170.4500) Optical coherence tomography medicine.diagnostic_test ocis:(110.5120) Photoacoustic imaging ocis:(170.2655) Functional monitoring and imaging Blood flow 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Volumetric flow rate chemistry 0210 nano-technology Biotechnology Biomedical engineering |
| Zdroj: | Biomedical Optics Express ResearcherID |
| ISSN: | 2156-7085 |
| Popis: | We proposed to measure the metabolic rate of oxygen (MRO(2)) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO(2) by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO(2)) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO(2) were further calculated. The measured blood flow rates in the two vessels agreed with each other. |
| Databáze: | OpenAIRE |
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