Proteome analysis of rat pancreatic acinar cells: Implication for cerulein-induced acute pancreatitis
| Autor: | Joo Weon Lim, Hyeyoung Kim, Shin Young Yun, Ji Hoon Yu, Kyung Hwan Kim |
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| Rok vydání: | 2003 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Cell signaling medicine.medical_specialty Protein subunit Molecular Sequence Data Biochemistry Heat shock protein Internal medicine medicine Animals Humans Electrophoresis Gel Two-Dimensional Amino Acid Sequence HSP90 Heat-Shock Proteins Pancreas Molecular Biology Cells Cultured biology Mitochondrial Proton-Translocating ATPases medicine.disease Molecular biology Mitochondria Rats Up-Regulation medicine.anatomical_structure Endocrinology Pancreatitis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Sulfurtransferases Digestive enzyme Proteome biology.protein Acute pancreatitis Reactive Oxygen Species Ceruletide |
| Zdroj: | PROTEOMICS. 3:2446-2453 |
| ISSN: | 1615-9861 1615-9853 |
| DOI: | 10.1002/pmic.200300545 |
| Popis: | Cerulein pancreatitis was shown to be one of the best characterized models for acute pancreatitis. High doses of cerulein induce a dysregulation of the digestive enzyme production and cytoplasmic vacuolization and the death of acinar cells, edema formation, and an infiltration of inflammatory cells into the pancreas, which are similar symptoms shown in human acute pancreatitis. The present study aims to determine the differentially expressed proteins in cerulein-treated pancreatic acinar cells as an in vitro model for acute pancreatitis. Pancreatic acinar AR42J cells were treated with 10 - 8 M cerulein for 24 h. The changed protein patterns separated by two-dimensional electrophoresis using pH gradients of 5-8 were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis of the peptide digests. Five differentially expressed proteins (heat shock protein 90, mitochondrial ATP synthase beta chain precursor, tubulin beta chain, 3-mercaptopyruvate sulfur-transferase, mitochondrial ATP synthase subunit D) were identified in cerulein-treated AR42J cells. These proteins are related to cellular stress such as reactive oxygen species, cytoskeletal function, and cell signaling. In conclusion, the differentially expressed proteins will provide valuable information to understand the pathophysiologic mechanism of acute pancreatitis and may be useful for prognostic indices of acute pancreatitis. |
| Databáze: | OpenAIRE |
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