Guidelines for Assessing Mouse Endothelial Function via Ultrasound Imaging: a Report from the International Society of Cardiovascular Translational Research
| Autor: | Zu-Yi Yuan, Wen Li, Jian Yang, Zhen Shan, Sudhanshu P. Raikwar, Shenming Wang, Chunxiang Zhang, Hua-ting Wang, Maoping Chu, Dan Yi, Jiaxin Zhan |
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| Rok vydání: | 2015 |
| Předmět: |
Carotid Artery Diseases
Male Apolipoprotein E Pathology medicine.medical_specialty Endothelium Carotid Artery Common Vasodilator Agents Carotid arteries Pharmaceutical Science Article Apolipoproteins E Predictive Value of Tests In vivo Genetics medicine Animals Genetics (clinical) Ultrasonography Mice Knockout Dose-Response Relationship Drug business.industry Ultrasound Reproducibility of Results Acetylcholine In vitro Disease Models Animal medicine.anatomical_structure Molecular Medicine Endothelium Vascular Cardiology and Cardiovascular Medicine business Function (biology) medicine.drug |
| Zdroj: | Journal of Cardiovascular Translational Research. 8:89-95 |
| ISSN: | 1937-5395 1937-5387 |
| DOI: | 10.1007/s12265-015-9614-8 |
| Popis: | The study is to establish a novel method to determine the endothelial function in mouse carotid arteries in vivo by using high-resolution ultrasound images. Atherosclerosis in carotid arteries is induced in ApoE−/− mice with a Western diet. The ultrasound of the ventral neck generates clear pictures of the common carotid arteries. Acetylcholine at the range from 5 to 20 μg/kg/min (iv) is able to induce a dose-dependent relaxation as shown by the increased diameter of these normal mouse carotid arteries, which is impaired in atherosclerotic arteries. The endothelial function determined by ultrasound images in vivo matches well with that determined in isolated carotid arterial rings in vitro. All animals are survival after the endothelial function measurement. In this study, we have established a standard method to determine the mouse endothelial function in vivo. It is a reliable, safe and survival method that could be used repetitively in mouse arteries. |
| Databáze: | OpenAIRE |
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