Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea
| Autor: | Jyh-Ching Chou, Jyun-De Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
citrate buffer
medicinal fungus Antrodia cinnamomea biology Regeneration (biology) fungi Immune regulation food and beverages Fungus protoplast regeneration Protoplast biochemical phenomena metabolism and nutrition biology.organism_classification Microbiology Citrate buffer lcsh:QK1-989 Infectious Diseases Biochemistry lcsh:Botany Osmotic pressure bacteria Mycelium Research Article protoplast preparation |
| Zdroj: | Mycobiology Mycobiology, Vol 47, Iss 4, Pp 483-493 (2019) |
| ISSN: | 2092-9323 1229-8093 |
| Popis: | Antrodia cinnamomea is a unique medicinal fungus in Taiwan. It has been found rich in some pharmacologically active compounds for anti-cancer, hangover, and immune regulation etc. With the in-depth study of these components, it would be interesting and important to establish a molecular system for basic studies of A. cinnamomea. Thus, we would like to set up a foundation for this purpose by studying the A. cinnamomea protoplast preparation and regeneration. Firstly, we studied the optimization method of protoplast preparation of A. cinnamomea, and found various factors that may affect the yield during protoplast preparation, such as mycelial ages, pH values, and osmotic stabilizers. Secondly, in the regeneration of protoplasts, we explored the effects of various conditions on the regeneration of protoplasts, including different media and osmotic pressure. In addition, we found that citrate buffer with pH value around 3 dramatically increased the regeneration of protoplasts of A. cinnamomea, and provided a set of regeneration methodology for A. cinnamomea. |
| Databáze: | OpenAIRE |
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