Screening and Use of High Titered Anti-HLA-DR Sera in PHA-Blast Complement Fixation and B-Lymphocytotoxicity Techniques
| Autor: | Jacques Colombani, Y. Gaudy, Jean Dausset, E. Terrier, Virginia Lepage |
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| Rok vydání: | 2008 |
| Předmět: |
Immunology
Biology Lymphocyte Activation Biochemistry Antibodies Epitopes Genetics HLA-DR Humans Immunology and Allergy Platelet Typing Phytohemagglutinins Cells Cultured B-Lymphocytes Immune Sera Complement Fixation Tests Histocompatibility Antigens Class II General Medicine Cytotoxicity Tests Immunologic Complement fixation test Peripheral blood Cell culture biology.protein Female Antibody |
| Zdroj: | Tissue Antigens. 17:37-42 |
| ISSN: | 0001-2815 |
| Popis: | Screening for anti-HLA-DR sera was performed by complement fixation on PHA stimulated peripheral blood lymphocytes (PHA-CF), or cultured B lymphoid cell lines. Out of 1,350 sera from multiparous women, multitransfused patients, and patients transfused during extra-corporal circulation (ECC), 219 contained anti-HLA-DR activity (16.2%). Anti-HLA-DR antibodies developed after ECC were often high titered (1:10 to 1:100). In half of these sera anti-HLA-A, B antibodies were weak or absent, making it possible to use then as anti-HLA-DR reagents without platelet absorption. Of the 219 positive sera 51 contained defined anti-DR antibodies (20 monospecific and 31 bi- or multispecific). The 13 best sera recognized DR1 and DR7 specificities with r values from 0.83 to 1. Twenty-four sera selected by CF were also studied by lymphocytotoxicity technique against peripheral blood B lymphocytes (B-LCT). Both PHA-CF and B-LCT techniques gave similar results, detecting the same specificities and showing comparable sensitivity. The advantages of CF are: easy storage of target cells at -80 degrees C or + 4 degrees C, and fast reading. For these reasons PHA-CF or CF on cultured B lymphoid cell lines can be proposed for large scale screening of anti-HLA-DR sera. The sera thus screened can be used for HLA-DR typing either by PHA-CF or B-LCT. |
| Databáze: | OpenAIRE |
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