CONSTRUCTION OF A TEST-SYSTEM WITH NANOPARTICLES OF COLLOID SILVER FOR DETECTION OF PSEUDOTUBERCULOSIS AND INTESTINAL YERSINIOSIS FOR CAUSATIVE AGENTS IN DOT-IMMUNOASSAY
| Autor: | Yu. O. Popova, M. V. Chesnokova, E. Yu. Markov, T. Yu. Zagoskina, O. A. Starikova, V. T. Klimov |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serotype Salmonella agglutinating specific sera 030231 tropical medicine 030106 microbiology Medicine (miscellaneous) Yersinia medicine.disease_cause Microbiology 03 medical and health sciences 0302 clinical medicine Shigella flexneri medicine Yersinia pseudotuberculosis Escherichia coli y. enterocolitica y. pseudotuberculosis medicine.diagnostic_test biology Chemistry Yersiniosis colloid silver General Medicine biology.organism_classification medicine.disease QR1-502 dot-immunoassay Immunoassay bacteria |
| Zdroj: | Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 1, Pp 55-61 (2017) |
| ISSN: | 2686-7613 0372-9311 |
| DOI: | 10.36233/0372-9311-2017-1-55-61 |
| Popis: | Aim. Construction of an immunologic test-system for detection of causative agents of entero-pathogenic yersinia {Yersinia pseudotuberculosis and Y. enterocolitica) by dot-immunoassay. Materials and methods. Nanoparticles of colloid silver sized 5 - 9 nm were used as a marker of specific antibodies. IgG fraction was isolated from commercial antisera to Y. pseudotuberculosis (0:1) and Y. enterocolitica (0:3 and 0:9). Testing of the obtained test-system was carried out on 20 strains of Y. pseudotuberculosis and Y. enterocolitica (10 of each species). Results. Dot-analysis had a specific character and detected enteropathogenic yersinia at a level of 5Т05 - 8T06 CFU/ml (100 - 1000 CFU in sample). Wherein cross-reaction with heterologic studied microorganisms - Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersiniapestis EV - was not observed. A possibility of simultaneous detection and serotyping of Y. enterocolitica is shown, that is necessary for confirmation of their epidemic significance. Conclusion. The developed test-systems allow to study micro volumes of the samples under study (1 pi), are express (1.5 - 2 h), highly sensitive and specific, technically simple and do not require the use of high-cost equipment, special training of the staff, may be successfully used in practical healthcare in laboratories with varying equipment levels. |
| Databáze: | OpenAIRE |
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