Unique Properties of a Cytotoxic CD4+CD8+Intraepithelial T-Cell Line Established from the Mouse Intestinal Epithelium
| Autor: | Hidekazu Tamauchi, Nobunao Ikewaki, Koichi Kubota, Takeshi Sasahara |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cytotoxicity
Immunologic Pore Forming Cytotoxic Proteins CD8 Antigens Receptors Antigen T-Cell alpha-beta T cell Immunology chemical and pharmacologic phenomena Biology Lymphocyte Activation digestive system Microbiology Cell Line Immunophenotyping Immunoenzyme Techniques Mice Intestinal mucosa Virology Intestine Small medicine Animals Cytotoxic T cell Intestinal Mucosa Cells Cultured Mice Inbred BALB C Membrane Glycoproteins Perforin T-cell receptor hemic and immune systems T lymphocyte Flow Cytometry Molecular biology medicine.anatomical_structure Cell culture CD4 Antigens Intraepithelial lymphocyte Female tissues CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Microbiology and Immunology. 38:191-199 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.1994.tb01764.x |
| Popis: | Growth factor-dependent gut intraepithelial lymphocyte (IEL) cell lines were established from a long-term in vitro culture of BALB/c IEL with syngeneic irradiated spleen cells in the presence of concanavalin A-stimulated spleen supernatant fluids. The cell lines were preferentially consisted of very limited thymoindependent subsets of IEL; i.e., Thy-1+CD5-TCR alpha beta+CD4+CD8 alpha+beta- (double-positive; DP) IEL and Thy-1+CD5-TCR alpha beta+CD4-CD8 alpha+beta- (CD8 single-positive; CD8 SP) IEL. The CD8 SP IEL cell line had cytotoxic activities and was triggered to proliferate by T-cell receptor (TCR)-directed stimuli. The DP IEL cell line expressed high levels of the CD3-TCR alpha beta, exhibited cytotoxic activity in redirected lysis assays, and had perforin in the cytoplasm, indicating the functional maturity of this cell line. However, the DP IEL cell line did not proliferate in response to TCR alpha beta-directed stimuli, which indicated that TCR alpha beta-mediated signalling was able to initiate cytotoxic function but not to induce proliferation of the DP IEL cell line. Although both cell lines were shown to have functional competence, they expressed J11d antigen which marks immaturity in thymocyte differentiation pathways. These results indicate that the established thymoindependent DP and CD8 SP IEL cell lines have unique properties distinct from DP thymocytes and CD8 SP peripheral T cells. Together with a recent report on freshly isolated DP IEL (10), the unique properties of the DP IEL cell line seems to support the notion that DP IEL may undergo a unique maturation process in the gut microenvironment. |
| Databáze: | OpenAIRE |
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