Interaction of Apoprotein from Porcine High-Density Lipoprotein with Dimyristoyl Lecithin. 1. The Structure of the Complexes
| Autor: | David Atkinson, H M Smith, J Dickson, J P Austin |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
food.ingredient
Morphology (linguistics) Materials science Protein Conformation Swine Phospholipid Biochemistry Lecithin chemistry.chemical_compound food X-Ray Diffraction Phosphatidylcholine Animals Dimyristoyl Lecithin Binding Sites Scattering Bilayer Crystallography chemistry X-ray crystallography Phosphatidylcholines lipids (amino acids peptides and proteins) Apoproteins Lipoproteins HDL Myristic Acids Protein Binding |
| Zdroj: | European Journal of Biochemistry. 64:541-547 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1976.tb10334.x |
| Popis: | The morphology and structural organisation of the complexes formed from the apoprotein of porcine high-density lipoprotein and dimyristoyl phosphatidylcholine (lecithin) have been studied using the technique of small-angle X-ray scattering. Scattering measurements made in solvents of varying electron density were interpreted in terms of a scattering-equivalent model for the structure of the complex. This model is described by an oblate ellipsoidal morphology with dimensions at 20 degrees C: major axis 11.0 nm, minor axis 5.5 nm. Within this overall shape the lipid hydrocarbon chains are organised in an apolar core whilst the lipid polar head groups and protein are located in a outer shell 0.85 nm in thickness. The oblate morphology demonstrates that the structure of the complex is directed by the fundamental bilayer organisation of the lecithin. The dimension of the minor axis (5.5 nm) indicates that phospholipid hydrocarbon chains are orientated perpendicular to the interface. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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