High Zinc Concentrations in Culture Media Affect Copper Uptake and Transport in Differentiated Human Colon Adenocarcinoma Cells
| Autor: | Mary Briske-Anderson, Samuel M. Newman, Philip G. Reeves |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Enterocyte
Medicine (miscellaneous) chemistry.chemical_element Zinc Intestinal mucosa medicine Humans Metallothionein Intestinal Mucosa Cadmium Nutrition and Dietetics Biological Transport Cell Differentiation Membrane transport Culture Media medicine.anatomical_structure Copper Radioisotopes chemistry Biochemistry Caco-2 Cell culture Caco-2 Cells Copper |
| Zdroj: | The Journal of Nutrition. 126:1701-1712 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/126.6.1701 |
| Popis: | Previous studies suggest that high dietary zinc reduces the copper status of animals by reducing copper transport across the intestinal mucosa. The present study used an enterocyte mimic, the Caco-2 cell, grown on porus membranes as a model to assess the effects of various concentrations of zinc (6 to 200 micromol/L) in the culture and assay media on 67Cu uptake and transport. Differentiated cells incubated for 7 d in a culture medium containing 50 micromol zinc + 0.7 micromol copper/L transported significantly less 67Cu across the monolayer than similar cells exposed to 6 micromol zinc/L. However, cells exposed to 200 micromol zinc/L for the same period transported significantly more 67Cu than those exposed to 6 micromol zinc/L. Cells exposed for only 1 h to 200 micromol zinc/L in the uptake-transport medium alone did not show lower 67Cu uptake or transport, suggesting that time of exposure of the cells to high zinc was a contributing factor. Caco-2 cells exposed to 50 through 200 micromol zinc/L had higher cellular metallothionein (MT) than those exposed to 6 micromol zinc/L. As the amount of MT in cells increased upon exposure to 50 and 100 micromol zinc/L, the rate of 67Cu transport decreased. At higher zinc concentrations in the medium, there was even more MT in the cells, but a greater rate Of 67Cu transport. These studies demonstrate the use of the Caco-2 cell as a model for copper uptake-transport studies, but the conditions must be rigorously defined and controlled. |
| Databáze: | OpenAIRE |
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