OCCURRENCE OF INOSINE KINASE AS A DISTINCT ENZYME IN SPIRULINA PLATENSIS
| Autor: | Maria Grazia Tozzi, D Barsacchi, Cosima Scolozzi, M Trinei, C Gualerzi, Pl Ipata |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Electrophoresis
Purine Biophysics Purine nucleoside phosphorylase macromolecular substances Biology Cyanobacteria Biochemistry chemistry.chemical_compound Inosine Monophosphate medicine heterocyclic compounds Inosine Molecular Biology Hypoxanthine Phosphorolysis Spirulina (genus) chemistry.chemical_classification Cell Biology biology.organism_classification Molecular biology Phosphotransferases (Alcohol Group Acceptor) enzymes and coenzymes (carbohydrates) Enzyme chemistry Purines bacteria Inosine kinase medicine.drug |
| Popis: | Among a series of purine nucleosides, inosine was found to be phosphorylated at the highest rate by crude extracts of the cyanobacterium Spirulina platensis . The inosine phosphorylating activity could be separated from hypoxanthine-guanine phosphoribosyl transferase. This result shows that IMP formation may occur via the direct phosphorylation of inosine at its 5′-position, rather than via inosine phosphorolysis, followed by hypoxanthine phosphoribosylation, and provides unequivocal evidence for the occurrence of inosine kinase in nature. |
| Databáze: | OpenAIRE |
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