Anti-Inflammatory and Free Radial Scavenging Activities of the Constituents Isolated from Machilus zuihoensis
| Autor: | Ih-Sheng Chen, Hsiang-Wen Tseng, Mei Hsien Lee, Shui-Tein Chen, Yi Wen Mao, Wen Li Liang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Interleukin-1beta Anti-Inflammatory Agents Pharmaceutical Science Nitric Oxide Synthase Type II Analytical Chemistry quercetin chemistry.chemical_compound Mice Superoxides Drug Discovery HMGB1 Protein anti-inflammatory chemistry.chemical_classification biology Molecular Structure Chemistry Superoxide Monosaccharides NF-kappa B Biological activity Free Radical Scavengers Interleukin-10 Nitric oxide synthase Biochemistry Chemistry (miscellaneous) Molecular Medicine Quercetin quercetin-3-O-β-D-glucopyranoside-(3¢→O-3¢¢¢)-quercetin-3-O-β-D-galactopyranoside ethyl caffeate Stereochemistry Article Nitric oxide Cell Line lcsh:QD241-441 Lauraceae Caffeic Acids lcsh:Organic chemistry Animals Humans quercetin-3-O-β-D-glucopyranoside-(3′→O-3‴)-quercetin-3-O-β-D-galactopyranoside Physical and Theoretical Chemistry Machilus zuihoensis Plant Extracts Tumor Necrosis Factor-alpha Macrophages Organic Chemistry Glycoside Ethyl caffeate Galactosides Quercitrin biology.protein |
| Zdroj: | Molecules Molecules; Volume 16; Issue 11; Pages: 9451-9466 Molecules, Vol 16, Iss 11, Pp 9451-9466 (2011) |
| ISSN: | 1420-3049 |
| Popis: | A new biflavonol glycoside, quercetin-3-O-β-D-glucopyranoside-(3¢→O-3¢¢¢)- quercetin-3-O-β-D-galactopyranoside (9), together with eight known compounds was isolated for the first time from the leaves of Machilus zuihoensis Hayata (Lauraceae). The structure of compound 9 was elucidated by various types of spectroscopic data analysis. Analysis of the biological activity assay found that compound 9 showed significant superoxide anion scavenging activity (IC50 is 30.4 μM) and markedly suppressed LPS-induced high mobility group box 1 (HMGB-1) protein secretion in RAW264.7 cells. In addition, the HMGB-1 protein secretion was also inhibited by quercitrin (3), ethyl caffeate (6), and ethyl 3-O-caffeoylquinate (7) treatment. In the LPS-stimulated inducible nitric oxide synthase (iNOS) activation analysis, two known compounds, quercetin (1) and ethyl caffeate (6), were found to markedly suppress nitric oxide (NO) production (IC50 value, 27.6 and 42.9 μM, respectively) in RAW264.7 cells. Additionally, it was determined that ethyl caffeate (6) down-regulated mRNA expressions of iNOS, IL-1β, and IL-10 in the LPS-treatment of RAW264.7 cells via a suppressed NF-kB pathway. These results suggested for the first time that the new compound 9 and other constituents isolated from M. zuihoensis have potential anti-inflammatory and superoxide anion scavenging effects. These constituents may be useful for treating various inflammatory diseases. |
| Databáze: | OpenAIRE |
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