The Human Telomere Sequence, (TTAGGG)4, in the Absence and Presence of Cosolutes: A Spectroscopic Investigation
| Autor: | Richard D. Sheardy, Vishal R Sharma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Conformational change
Circular dichroism Osmotic shock Stereochemistry Potassium Compounds Pharmaceutical Science Polyethylene glycol Article Analytical Chemistry Molecularity Phosphates Polyethylene Glycols lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry Potassium phosphate Osmotic Pressure Drug Discovery Humans DNA quadruplexes circular dichroism PEG osmotic stress Physical and Theoretical Chemistry chemistry.chemical_classification Base Sequence Biomolecule Organic Chemistry Telomere G-Quadruplexes chemistry Chemistry (miscellaneous) Nucleic acid Biophysics Molecular Medicine Nucleic Acid Conformation Thermodynamics |
| Zdroj: | Molecules, Vol 19, Iss 1, Pp 595-608 (2014) Molecules; Volume 19; Issue 1; Pages: 595-608 Molecules |
| ISSN: | 1420-3049 |
| Popis: | Historically, biophysical studies of nucleic acids have been carried out under near ideal conditions, i.e., low buffer concentration (e.g., 10 mM phosphate), pH 7, low ionic strength (e.g., 100 mM) and, for optical studies, low concentrations of DNA (e.g., 1 × 10−6 M). Although valuable structural and thermodynamic data have come out of these studies, the conditions, for the most, part, are inadequate to simulate realistic cellular conditions. The increasing interest in studying biomolecules under more cellular-like conditions prompted us to investigate the effect of osmotic stress on the structural and thermodynamic properties of DNA oligomers containing the human telomere sequence (TTAGGG). Here, we report the characterization of (TTAGGG)4 in potassium phosphate buffer with increasing percent PEG (polyethylene glycol) or acetonitrile. In general, the presence of these cosolutes induces a conformational change from a unimolecular hybrid structure to a multimolecular parallel stranded structure. Hence, the structural change is accompanied with a change in the molecularity of quadruplex formation. |
| Databáze: | OpenAIRE |
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