A sensitive radioenzymatic assay for dihdyroxymandelic acid, dihydroxyphenylglycol and dihydroxyphenylacetic acid
| Autor: | M. Nagel-Hiemke, H.J. Schümann |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Radioisotope Dilution Technique
S-Adenosylmethionine Biophysics Catechol O-Methyltransferase Tritium Biochemistry Methoxyhydroxyphenylglycol Glycols chemistry.chemical_compound Heart perfusion Dopamine medicine Humans Phenylacetates Methionine Chromatography Dihydroxyphenylacetic acid Organic solvent Extraction (chemistry) Periodate Kinetics chemistry 3 4-Dihydroxyphenylacetic Acid Mandelic Acids Methyl donor medicine.drug |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 4:261-270 |
| ISSN: | 0165-022X |
| DOI: | 10.1016/0165-022x(81)90066-x |
| Popis: | A sensitive radioenzymatic procedure for the simultaneous determination of dihydroxymandelic acid, dihydroxyphenylglycol, and dihydroxyphenylacetic acid has been developed. The catechols were methylated by catechol-O-methyltransferase using S-adenosyl[methyl-3H]methionine as methyl donor. The assay involved extraction into organic solvent, thin-layer chromatography and periodate oxidation. The sensitivity of the assay was better than 15 pg per sample. The applicability of the procedure was tested by measuring the amounts of deaminated metabolites of noradrenaline, adrenaline and dopamine in heart perfusion samples and plasma of rabbits. |
| Databáze: | OpenAIRE |
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