Schwann cells differentiated from spheroid-forming cells of rat subcutaneous fat tissue myelinate axons in the spinal cord injury

Autor: Guang Fan Chi, Dae-Wook Kim, Mei Hua Jiang, Youngsook Son, Mi-Ra Kim
Rok vydání: 2010
Předmět:
Male
Cell Adhesion Molecules
Neuronal
Green Fluorescent Proteins
Adipose tissue
Schwann cell
Nerve Tissue Proteins
Biology
Receptor
Nerve Growth Factor
Animals
Genetically Modified
Nestin
Rats
Sprague-Dawley
Myelin
Intermediate Filament Proteins
Developmental Neuroscience
Neurofilament Proteins
Neurotrophic factors
Gangliosides
Spheroids
Cellular
Glial Fibrillary Acidic Protein
medicine
Animals
Axon
Cells
Cultured
Early Growth Response Protein 2
Spinal Cord Injuries
SOXE Transcription Factors
Multipotent Stem Cells
S100 Proteins
Hematopoietic Stem Cell Transplantation
Cell Differentiation
Rats
Cell biology
Disease Models
Animal
medicine.anatomical_structure
nervous system
Neurology
Peripheral nervous system
Immunology
Neuroglia
Schwann Cells
Stem cell
Leukocyte L1 Antigen Complex
Myelin P0 Protein
Zdroj: Experimental Neurology. 222:304-317
ISSN: 0014-4886
DOI: 10.1016/j.expneurol.2010.01.008
Popis: In the present study, we found that nestin-expressing spheroid cells derived from multipotent adipose stem cells of subcutaneous fat tissue could efficiently differentiate into Schwann cells (SCs) in vitro based on expression of SC markers such as A2B5, GFAP, O4, p75, S100, Sox10, Krox-20, and L1. The induced SC is engrafted to spinal cord injury lesions and formed a peripheral nervous system (PNS)-type myelin sheath on central nervous system (CNS) axons. PNS-type myelin sheath formation in repaired tissue was confirmed by transplantation of both induced PKH26-labeled SC and induced EGFP-expressing SC generated from EGFP transgenic rats. In addition to direct participation as myelin sheath-forming SC in repaired tissue, the induced SC also expressed several neurotrophic factors, as did native SC, which may suggest an additional role for induced SC in stimulation of endogenous healing responses. Thus, spheroid-forming cells from subcutaneous fat tissue demonstrated rapid and efficient induction into SC, and such cells show therapeutic promise for repair of damage to the CNS and PNS.
Databáze: OpenAIRE
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