Identification of Differentially Expressed Genes during Bacillus subtilis Spore Outgrowth in High-Salinity Environments Using RNA Sequencing
| Autor: | Tamara Hoffmann, Oscar P. Kuipers, Erhard Bremer, Anne de Jong, Kazimierz Madela, Katja Nagler, Ralf Moeller, Antonina O. Krawczyk, Michael Laue |
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| Přispěvatelé: | Molecular Genetics |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Osmotic shock 030106 microbiology lcsh:QR1-502 high salinity Bacillus subtilis Biology Microbiology lcsh:Microbiology Transcriptome Strahlenbiologie 03 medical and health sciences B.subtilis NaCl Gene expression Spore germination B. subtilis spore germination Original Research fungi biology.organism_classification ripening Cell biology Spore spore germination 030104 developmental biology Regulon B subtilis spore germination outgrowth Osmoprotectant osmotic stress RNA-seq |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 7 (2016) Frontiers in Microbiology, 7:1564, 1-15. Frontiers Media S.A. |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2016.01564 |
| Popis: | In its natural habitat, the soil bacterium Bacillus subtilis often has to cope with fluctuating osmolality and nutrient availability. Upon nutrient depletion it can form dormant spores, which can revive to form vegetative cells when nutrients become available again. While the effects of salt stress on spore germination have been analyzed previously, detailed knowledge on the salt stress response during the subsequent outgrowth phase is lacking. In this study, we investigated the changes in gene expression during B. subtilis outgrowth in the presence of 1.2 M NaCl using RNA sequencing. In total, 402 different genes were upregulated and 632 genes were downregulated during 90 min of outgrowth in the presence of salt. The salt stress response of outgrowing spores largely resembled the osmospecific response of vegetative cells exposed to sustained high salinity and included strong upregulation of genes involved in osmoprotectant uptake and compatible solute synthesis. The σ(B)-dependent general stress response typically triggered by salt shocks was not induced, whereas the σ(W) regulon appears to play an important role for osmoadaptation of outgrowing spores. Furthermore, high salinity induced many changes in the membrane protein and transporter transcriptome. Overall, salt stress seemed to slow down the complex molecular reorganization processes ("ripening") of outgrowing spores by exerting detrimental effects on vegetative functions such as amino acid metabolism. |
| Databáze: | OpenAIRE |
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