Prevention of Adult Colitis by Oral Ferric Iron in Juvenile Mice Is Associated with the Inhibition of the Tbet Promoter Hypomethylation and Gene Overexpression
Autor: | Moïse Coëffier, Carine Delayre-Orthez, Abalo Chango, Chourouk Ettreiki, Pauline M. Anton, Nicolas Barbezier |
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Přispěvatelé: | UniLaSalle, Nutrition, inflammation et dysfonctionnement de l'axe intestin-cerveau (ADEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Normandie Université (NU), Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de nutrition [CHU Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Transformations et Agro-ressources (UT&A), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANTON, Pauline, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Male Aging Administration Oral Ferric Compounds Mice 0302 clinical medicine Gene expression intestinal inflammation Medicine Ingestion Promoter Regions Genetic Mice Inbred BALB C Nutrition and Dietetics GATA3 FOXP3 Methylation Colitis 3. Good health medicine.anatomical_structure 030220 oncology & carcinogenesis [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] lcsh:Nutrition. Foods and food supply Tbet Adult medicine.medical_specialty Drug Compounding Spleen lcsh:TX341-641 promoter methylation Article 03 medical and health sciences Internal medicine [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Animals Humans Transcription factor business.industry ferric iron [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology medicine.disease [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition 030104 developmental biology Endocrinology Gene Expression Regulation Th profile CpG Islands business [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Food Science Transcription Factors |
Zdroj: | Nutrients Volume 11 Issue 8 Nutrients, MDPI, 2019, 11 (8), ⟨10.3390/nu11081758⟩ Nutrients, 2019, 11 (8), pp.1758. ⟨10.3390/nu11081758⟩ Nutrients, Vol 11, Iss 8, p 1758 (2019) Nutrients, MDPI, 2019, 11 (8), pp.1758. ⟨10.3390/nu11081758⟩ |
ISSN: | 2072-6643 |
DOI: | 10.3390/nu11081758 |
Popis: | Iron is an essential nutrient needed for physiological functions, particularly during the developmental period of the early childhood of at-risk populations. The purpose of this study was to investigate, in an experimental colitis, the consequences of daily oral iron ingestion in the early period on the inflammatory response, the spleen T helper (Th) profiles and the associated molecular mechanisms. Juvenile mice orally received microencapsulated ferric iron or water for 6 weeks. On adult mice, we induced a sham or experimental trinitrobenzene sulfonic acid (TNBS) moderate colitis during the last week of the experiment before sacrificing the animals 7 days later. The severity of the gut inflammation was assessed by macroscopic damage scores (MDS) and the myeloperoxidase activity (MPO). Th profiles were evaluated by the examination of the splenic gene expression of key transcription factors of the Th differentiation (Tbet, Gata3, Foxp3 and ROR&gamma ) and the methylation of their respective promoter. While TNBS-induced colitis was associated with a change of the Th profile (notably an increase in the Tbet/Gata3 ratio in the spleen), the colitis-inhibition induced by ferric iron was associated with a limitation of the splenic Th profiles perturbation. The inhibition of the splenic Tbet gene overexpression was associated with an inhibition of promoter hypomethylation. In summary, mice treated by long-term oral ferric iron in the early period of life exhibited an inhibition of colitis associated with the inhibition of the splenic Tbet promoter hypomethylation and gene overexpression. |
Databáze: | OpenAIRE |
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