Prevention of Adult Colitis by Oral Ferric Iron in Juvenile Mice Is Associated with the Inhibition of the Tbet Promoter Hypomethylation and Gene Overexpression

Autor: Moïse Coëffier, Carine Delayre-Orthez, Abalo Chango, Chourouk Ettreiki, Pauline M. Anton, Nicolas Barbezier
Přispěvatelé: UniLaSalle, Nutrition, inflammation et dysfonctionnement de l'axe intestin-cerveau (ADEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Normandie Université (NU), Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de nutrition [CHU Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Transformations et Agro-ressources (UT&A), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANTON, Pauline, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Aging
Administration
Oral

Ferric Compounds
Mice
0302 clinical medicine
Gene expression
intestinal inflammation
Medicine
Ingestion
Promoter Regions
Genetic

Mice
Inbred BALB C

Nutrition and Dietetics
GATA3
FOXP3
Methylation
Colitis
3. Good health
medicine.anatomical_structure
030220 oncology & carcinogenesis
[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]

lcsh:Nutrition. Foods and food supply
Tbet
Adult
medicine.medical_specialty
Drug Compounding
Spleen
lcsh:TX341-641
promoter methylation
Article
03 medical and health sciences
Internal medicine
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]

Animals
Humans
Transcription factor
business.industry
ferric iron
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
medicine.disease
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
030104 developmental biology
Endocrinology
Gene Expression Regulation
Th profile
CpG Islands
business
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Food Science
Transcription Factors
Zdroj: Nutrients
Volume 11
Issue 8
Nutrients, MDPI, 2019, 11 (8), ⟨10.3390/nu11081758⟩
Nutrients, 2019, 11 (8), pp.1758. ⟨10.3390/nu11081758⟩
Nutrients, Vol 11, Iss 8, p 1758 (2019)
Nutrients, MDPI, 2019, 11 (8), pp.1758. ⟨10.3390/nu11081758⟩
ISSN: 2072-6643
DOI: 10.3390/nu11081758
Popis: Iron is an essential nutrient needed for physiological functions, particularly during the developmental period of the early childhood of at-risk populations. The purpose of this study was to investigate, in an experimental colitis, the consequences of daily oral iron ingestion in the early period on the inflammatory response, the spleen T helper (Th) profiles and the associated molecular mechanisms. Juvenile mice orally received microencapsulated ferric iron or water for 6 weeks. On adult mice, we induced a sham or experimental trinitrobenzene sulfonic acid (TNBS) moderate colitis during the last week of the experiment before sacrificing the animals 7 days later. The severity of the gut inflammation was assessed by macroscopic damage scores (MDS) and the myeloperoxidase activity (MPO). Th profiles were evaluated by the examination of the splenic gene expression of key transcription factors of the Th differentiation (Tbet, Gata3, Foxp3 and ROR&gamma
) and the methylation of their respective promoter. While TNBS-induced colitis was associated with a change of the Th profile (notably an increase in the Tbet/Gata3 ratio in the spleen), the colitis-inhibition induced by ferric iron was associated with a limitation of the splenic Th profiles perturbation. The inhibition of the splenic Tbet gene overexpression was associated with an inhibition of promoter hypomethylation. In summary, mice treated by long-term oral ferric iron in the early period of life exhibited an inhibition of colitis associated with the inhibition of the splenic Tbet promoter hypomethylation and gene overexpression.
Databáze: OpenAIRE