FertDish: microfluidic sperm selection-in-a-dish for intracytoplasmic sperm injection
| Autor: | David Sinton, Sa Xiao, Alexander Lagunov, Mohammad Simchi, Thomas Hannam, Keith Jarvi, Reza Nosrati, Jason Riordon |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system medicine.medical_treatment Microfluidics Biomedical Engineering Bioengineering Semen Biology Biochemistry Intracytoplasmic sperm injection law.invention Andrology 03 medical and health sciences 0302 clinical medicine law medicine Humans Sperm Injections Intracytoplasmic reproductive and urinary physiology Selection (genetic algorithm) 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine urogenital system Petri dish General Chemistry Limiting Spermatozoa Sperm embryonic structures Selection method |
| Zdroj: | Lab on a Chip. 21:775-783 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | The selection of high quality sperm is critical for intracytoplasmic sperm injection (ICSI), a prevalent assisted reproduction technology. However, standard selection methods are time-consuming and fail to recover the most viable sperm, thereby limiting the ICSI success rate. Microfluidics enables rapid selection of viable sperm in a manner representing in vivo processes, however, existing platforms lack clinical applicability. Here, we present FertDish, which integrates the clinically established ICSI Petri dish with a film featuring an array of sperm-selecting microchannels for selection of sperm directly from semen. The FertDish format mimics the clinician-familiar ICSI dish setup, and provides rapid (10 min) single stage sperm preparation that circumvents standard labour-intensive multi-stage sperm processing steps. Tests with human donor and patient semen samples show that FertDish enables the selection of a high quality sperm sub-population, featuring improvements in DNA fragmentation index of more than 91% (donor) and 74% (patient) versus raw semen and 50% (donor) and 63% (patient) versus standard methods, and a distribution of more than 97% sperm with viable and high level DNA. The FertDish enables a high sperm recovery rate (3.3 × 105 sperm per mL), and is readily adaptable to the clinical workflow with potential to improve ICSI outcomes. |
| Databáze: | OpenAIRE |
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