Human intron-encoded AluACA RNAs and telomerase RNA share a common element promoting RNA accumulation
| Autor: | Amandine Ketele, Beáta E. Jády, Tamás Kiss |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Models Molecular H/ACA RNP biogenesis Gene Expression Biology Dyskerin 03 medical and health sciences Telomerase RNA component 0302 clinical medicine Box H/ACA RNAs Humans Small nucleolar RNA AluACA RNAs Molecular Biology Telomerase intronic RNA processing Intron RNA Cell Biology Telomere Non-coding RNA Molecular biology Long non-coding RNA Introns H/ACA RNA processing RNA silencing 030104 developmental biology Nucleic Acid Conformation telomerase RNA RNA Editing RNA Polymerase II 030217 neurology & neurosurgery Research Paper HeLa Cells |
| Zdroj: | RNA Biology |
| ISSN: | 1555-8584 1547-6286 |
| Popis: | Mammalian cells express hundreds of intron-encoded box H/ACA RNAs which fold into a common hairpin-hinge-hairpin-tail structure, interact with 4 evolutionarily conserved proteins, dyskerin, Nop10, Nhp2 and Gar1, and function mainly in RNA pseudouridylation. The human telomerase H/ACA RNA (hTR) directs telomeric DNA synthesis and it carries a 5′-terminal domain encompassing the telomeric template sequence. The primary hTR transcript is synthesized from an independent gene by RNA polymerase II and undergoes 3′ end processing controlled by the 3′-terminal H/ACA domain. The apical stem-loop of the 3′ hairpin of hTR carries a unique biogenesis-promoting element, the BIO motif that promotes hTR processing and RNP assembly. AluACA RNAs represent a distinct class of human H/ACA RNAs; they are processed from intronic Alu repetitive sequences. As compared to canonical H/ACA RNAs, the AluACA RNAs carry unusually short or long 5′ hairpins and generally, they accumulate at low levels. Here, we demonstrate that the suboptimal 5′ hairpins are responsible for the weak expression of AluACA RNAs. We also show that AluACA RNAs frequently carry a processing/stabilization element that is structurally and functionally indistinguishable from the hTR BIO motif. Both hTR and AluACA biogenesis-promoting elements are located in the terminal stem-loop of the 3′-terminal H/ACA hairpin, they show perfect structural conservation and are functionally interchangeable in in vivo RNA processing reactions. Our results demonstrate that the BIO motif, instead of being confined to hTR, is a more general H/ACA RNP biogenesis-facilitating element that can also promote processing/assembly of intron-encoded AluACA RNPs. |
| Databáze: | OpenAIRE |
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