HIF-1α inhibits mitochondria-mediated apoptosis and improves the survival of human adipose-derived stem cells in ischemic microenvironments
| Autor: | Hao Wu, Tianqi Lv, Jiayi Xu, Giorgi Oganezov, Hao Pang, Jie Wang, Xiaoqun Dong, Ying Liu, Jiaxu Li, Yongting Zhou, Zhibo Xiao |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_specialty Cell Survival Adipose tissue Apoptosis In Vitro Techniques 030204 cardiovascular system & hematology Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ischemia medicine Humans Cell Proliferation TUNEL assay medicine.diagnostic_test business.industry Mesenchymal stem cell Hypoxia-Inducible Factor 1 alpha Subunit Mitochondria Surgery Vascular endothelial growth factor Transplantation 030104 developmental biology Adipose Tissue chemistry Cancer research Female Stem cell business Stem Cell Transplantation |
| Zdroj: | Journal of Plastic, Reconstructive & Aesthetic Surgery. 74:1908-1918 |
| ISSN: | 1748-6815 |
| Popis: | Summary Background Human adipose mesenchymal stem cells (hADSCs) show poor survival after transplantation, limiting their clinical application. Tissue regeneration resulting from stem cell treatment may be caused by attenuation of hypoxia-inducible factor-1α (HIF-1α). In this study, we constructed hADSCs stably expressing HIF-1α and investigated the potential effects of HIF-1α expression in the ischemic microenvironment on mitochondrial apoptosis and survival of hADSCs, and studied the mechanisms involved. Method Apoptosis was induced by an ischemic microenvironment in vitro. ADSCs with stable HIF-1α expression were established. Cell survival and apoptosis were observed by CCK-8 assay, western blotting, flow cytometry, and fluorescence staining. ADSCs were subcutaneously transplanted into nude mice in the location where a hypoxia ischemic microenvironment was simulated in vivo. After 1, 3, and 7 d, mitochondrial apoptotic proteins were evaluated by immunohistochemistry and immunofluorescence staining. Results Exogenous HIF-1α downregulated mitochondrial reactive oxygen species, cytochrome c, caspase-9, and caspase-3, but inhibited mitochondrial membrane potential depolarization and increased the Bcl-2/bax ratio. HIF-1α prevented apoptosis and promoted vascular endothelial growth factor (VEGF) secretion as demonstrated by enzyme-linked immunosorbent assay (ELISA), terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, and flow cytometry analysis. HIF-1α enhanced the survival of transplanted ADSCs in nude mice. Conclusion We have shown that through inhibition of the mitochondria-mediated apoptotic pathway and promotion of VEGF secretion in hADSCs in an ischemic microenvironment, HIF-1α may potentially be applied in clinical therapy and as an alternative strategy for improving hADSC therapy. |
| Databáze: | OpenAIRE |
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