Characterization of a genomic region encoding the 32-kDa dense granule antigen of Sarcocystis muris (Apicomplexa)
| Autor: | Bernd Freyer, Thomas Hansner, Wolfgang Rüger, Heinz Mehlhorn |
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| Rok vydání: | 1999 |
| Předmět: |
Sarcocystosis
Genes Protozoan Molecular Sequence Data Protozoan Proteins Antigens Protozoan Biology Polymerase Chain Reaction Mice Exon Complementary DNA Consensus sequence Animals Coding region Amino Acid Sequence Genomic organization Genetics Mice Inbred BALB C Base Sequence General Veterinary Intron Nucleic acid sequence Sarcocystis Sequence Analysis DNA General Medicine DNA Protozoan Molecular biology Open reading frame Infectious Diseases Insect Science Parasitology |
| Zdroj: | Parasitology Research. 85:923-927 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Two subgenomic libraries constructed from Sarcocystis muris total DNA were screened with a cDNA probe, specific for a 32-kDa protein associated with the dense granules. Two clones reacted positively and were isolated, gDG 32/1 and gDG 32/2. Genomic clone gDG32/1 and part of clone gDG 32/2 have been sequenced. The composite nucleotide sequence of these genomic clones comprises 4.34 kb. It contains a 5' region of 2.14 kb, a first coding region (222 bp, exon I), a noncoding region (608 bp, intron), a second coding region (660 bp, exon II), and a 3' region of 693 bp. The upstream region shows a eukaryotic promoter structure and a consensus sequence for the 5' and 3' splicing sites. Thus the open reading frame (ORF DG32) coding for the 32-kDa protein of the dense granules of S. muris cyst merozoites is interrupted by an intron. To our knowledge, dg32 is the first sarcosporidian mosaic gene to be characterized. |
| Databáze: | OpenAIRE |
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