Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

Autor: Lin Li, Lanying Du, Yuxian He, Guangyu Zhao, Yusen Zhou, Shibo Jiang, Bo-Jian Zheng
Rok vydání: 2009
Předmět:
Stable expression
T-Lymphocytes
medicine.disease_cause
Antibodies
Viral
Severe Acute Respiratory Syndrome
Biochemistry
Epitope
Mice
0302 clinical medicine
Viral Envelope Proteins
Viral Envelope Proteins - biosynthesis - immunology
Cricetinae
Neutralizing antibody
Coronavirus
0303 health sciences
Mice
Inbred BALB C
Membrane Glycoproteins
Immunogenicity
Vaccination
CHO cells
SARS-CoV
Antigenicity
3. Good health
Severe acute respiratory syndrome-related coronavirus
Spike Glycoprotein
Coronavirus
Receptors
Virus
Female
Antibody
Binding domain
medicine.drug_class
Biophysics
Biology
Monoclonal antibody
Article
SARS Virus - immunology
S protein
03 medical and health sciences
Cricetulus
medicine
Animals
Molecular Biology
030304 developmental biology
Cell Biology
Virology
Molecular biology
Receptor-binding domain
Protein Structure
Tertiary
Membrane Glycoproteins - biosynthesis - immunology
Antibodies
Viral - biosynthesis
biology.protein
Interleukin-4
030215 immunology
Zdroj: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2009.05.003
Popis: The receptor-binding domain (RBD) of SARS coronavirus (SARS-CoV) spike (S) protein contains multiple conformation-dependent epitopes that induce neutralizing antibody responses. Here we used CHO-K1 cells to establish a cell line for stable expression of a 193-mer (residues 318-510) RBD (RBD193-CHO) and determined its antigenicity and immunogenicity. We found that RBD193-CHO reacted strongly with a panel of six monoclonal antibodies recognizing various conformational and linear epitopes in RBD, suggesting that this recombinant protein maintains intact conformation and good antigenicity. Immunization of mice with RBD193-CHO resulted in induction of high titers of RBD-specific neutralizing antibodies and potent IL-4-expressing T cell responses. RBD193-CHO induced immunity that protected a majority of the vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD produced in an established stable cell line maintains strong immunogenicity with high potential for use as an effective and economic subunit SARS vaccine. © 2009 Elsevier Inc. All rights reserved.
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