Unique butyric acid incorporation patterns for salinosporamides A and B reveal distinct biosynthetic origins
| Autor: | Kin S. Lam, Katherine A. McArthur, Barbara C. M. Potts, Ginger Tsueng |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Magnetic Resonance Spectroscopy
Lactams Stereochemistry Sodium chemistry.chemical_element Butyrate Applied Microbiology and Biotechnology Butyric acid chemistry.chemical_compound Lactones Biosynthesis Liquid chromatography–mass spectrometry mental disorders Pyrroles biology Sodium butyrate Micromonosporaceae General Medicine biology.organism_classification humanities Biochemistry chemistry Salinispora tropica Butyric Acid Salinosporamide A Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 75(5) |
| ISSN: | 0175-7598 |
| Popis: | Feeding sodium butyrate (0.25-1 mg/ml) to cultures of Salinispora tropica NPS21184 enhanced the production of salinosporamide B (NPI-0047) by 319% while inhibiting the production of salinosporamide A (NPI-0052) by 26%. Liquid chromatography mass spectrometry analysis of the crude extract from the strain NPS21184 fed with 0.5 mg/ml sodium [U-(13)C(4)]butyrate indicated that butyrate was incorporated as a contiguous four-carbon unit into NPI-0047 but not into NPI-0052. Nuclear magnetic resonance analysis of NPI-0047 and NPI-0052 purified from the sodium [U-(13)C(4)]butyrate-supplemented culture extract confirmed this incorporation pattern. The above finding is the first direct evidence to demonstrate that the biosynthesis of NPI-0047 is different from NPI-0052, and NPI-0047 is not a precursor of NPI-0052. |
| Databáze: | OpenAIRE |
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