Jasmonate Controls Leaf Growth by Repressing Cell Proliferation and the Onset of Endoreduplication while Maintaining a Potential Stand-By Mode
| Autor: | Takashi Ishida, Hugh P. Shanahan, Moritz Bömer, Sandra Noir, Alessandra Devoto, Virginia Balbi, Keiko Sugimoto, Tjir-Li Tsui, Naoki Takahashi |
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| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Department for Molecular Biomedical Research, Vib Ghent University |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Cell division Physiology [SDV.BC]Life Sciences [q-bio]/Cellular Biology Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Mitotic cell cycle Arabidopsis Genetics Endoreduplication Arabidopsis thaliana Jasmonate ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Methyl jasmonate Cell growth food and beverages [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology biology.organism_classification Cell biology chemistry 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2013, 161 (4), pp.1930-1951 |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | Phytohormones regulate plant growth from cell division to organ development. Jasmonates (JAs) are signaling molecules that have been implicated in stress-induced responses. However, they have also been shown to inhibit plant growth, but the mechanisms are not well understood. The effects of methyl jasmonate (MeJA) on leaf growth regulation were investigated in Arabidopsis (Arabidopsis thaliana) mutants altered in JA synthesis and perception, allene oxide synthase and coi1-16B (for coronatine insensitive1), respectively. We show that MeJA inhibits leaf growth through the JA receptor COI1 by reducing both cell number and size. Further investigations using flow cytometry analyses allowed us to evaluate ploidy levels and to monitor cell cycle progression in leaves and cotyledons of Arabidopsis and/or Nicotiana benthamiana at different stages of development. Additionally, a novel global transcription profiling analysis involving continuous treatment with MeJA was carried out to identify the molecular players whose expression is regulated during leaf development by this hormone and COI1. The results of these studies revealed that MeJA delays the switch from the mitotic cell cycle to the endoreduplication cycle, which accompanies cell expansion, in a COI1-dependent manner and inhibits the mitotic cycle itself, arresting cells in G1 phase prior to the S-phase transition. Significantly, we show that MeJA activates critical regulators of endoreduplication and affects the expression of key determinants of DNA replication. Our discoveries also suggest that MeJA may contribute to the maintenance of a cellular “stand-by mode” by keeping the expression of ribosomal genes at an elevated level. Finally, we propose a novel model for MeJA-regulated COI1-dependent leaf growth inhibition. |
| Databáze: | OpenAIRE |
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