Primary structure, gene expression and chromosomal mapping of rodent homologs of the MEN1 tumor suppressor gene
| Autor: | Henning Dralle, Hans Michael Dosch, Sabine Maier, Bernhard O. Boehm, Andrea Wissmann, Wolfram Karges |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities endocrine system DNA Complementary endocrine system diseases Tumor suppressor gene Molecular Sequence Data Biophysics Gene mutation Biology medicine.disease_cause Biochemistry Mice Peptide Library Structural Biology Sequence Homology Nucleic Acid Complementary DNA Gene expression Multiple Endocrine Neoplasia Type 1 Tumor Cells Cultured Genetics medicine Animals Humans Genes Tumor Suppressor MEN1 RNA Messenger Rats Wistar Multiple endocrine neoplasia Gene In Situ Hybridization Fluorescence Reverse Transcriptase Polymerase Chain Reaction Chromosome Mapping medicine.disease Molecular biology Rats Gene Expression Regulation DNA Probes Carcinogenesis Transcription Factors |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1446:286-294 |
| ISSN: | 0167-4781 |
| Popis: | Mutations of the MEN1 tumor suppressor gene cause the multiple endocrine neoplasia type 1 (MEN1) syndrome in humans, and they are involved in a variety of sporadic human endocrine tumors. We here characterize the MEN1 gene homologs of the mouse and rat. cDNA was isolated from a mouse phage library, and two alternative MEN1 mRNA transcripts containing variant 5′ untranslated regions were identified by RT-PCR in several mouse and rat tissues. When compared to the human molecule, mouse and rat MEN1 (611 and 610 amino acids, respectively) show an overall identity of 96.5% and 97.0% at the protein level, delimiting four conservational domains (A–D). Mouse and rat MEN1 mRNA, as studied by template-calibrated quantitative RT-PCR, is non-exclusively expressed in hematopoietic and endocrine cells, with similar expression patterns found in fetal and adult tissues. Fluorescent in situ hybridization maps the single murine MEN1 locus to chromosome 19, region B. No MEN1 gene mutations were identified in endocrine islet tumor cell lines RIN 5AH (rat) and NIT-1 (mouse) as compared to wild type cDNA. Our data define mouse and rat MEN1 as widely expressed and highly conserved homologs of the human MEN1 tumor suppressor gene whose role in biology and endocrine tumorigenesis is due for experimental study. |
| Databáze: | OpenAIRE |
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