Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis
| Autor: | Rodrigo Zuloaga, Herman Silva, Sebastian Naour, Jorge E. Aedo, Alfredo Molina, Juan Antonio Valdés, Claudio Meneses, Macarena Bastias-Molina, Brisa M. Espinoza, Jonathan Maldonado, Cristian Gallardo-Escárate |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nonalcoholic fatty liver Gene Expression lcsh:Medicine 010501 environmental sciences medicine.disease_cause Biochemistry 01 natural sciences Non-alcoholic Fatty Liver Disease Medicine and Health Sciences Glycolysis lcsh:Science Cellular Stress Responses Multidisciplinary Liver Diseases Fatty Acids Fatty liver Fishes Sequence analysis Genomics Lipids Gene expression profiling Cholesterol Liver Cell Processes Alkaline phosphatase Transcriptome Analysis Research Article medicine.medical_specialty endocrine system animal structures Gastroenterology and Hepatology Biology Biosynthesis 03 medical and health sciences Internal medicine medicine Genetics Animals 14. Life underwater 0105 earth and related environmental sciences Sequence Analysis RNA Catabolism Animal Gene Expression Profiling lcsh:R Biology and Life Sciences Computational Biology Lipid metabolism Cell Biology Metabolism Genome Analysis Lipid Metabolism medicine.disease Fatty Liver Oxidative Stress Gene Ontology 030104 developmental biology Endocrinology Fish Gluconeogenesis Oxidative stress lcsh:Q Gene ontology |
| Zdroj: | PLoS One Artículos CONICYT CONICYT Chile instacron:CONICYT PLoS ONE, Vol 12, Iss 4, p e0176447 (2017) PLoS ONE |
| Popis: | Indexación: Scopus. Teleosts exhibit a broad divergence in their adaptive response to stress, depending on the magnitude, duration, and frequency of stressors and the species receiving the stimulus. We have previously reported that the red cusk-eel (Genypterus chilensis), an important marine farmed fish, shows a physiological response to stress that results in increased skeletal muscle atrophy mediated by over-expression of components of the ubiquitin proteasome and autophagy-lysosomal systems. To better understand the systemic effects of stress on the red cusk-eel metabolism, the present study assessed the transcriptomic hepatic response to repetitive handling-stress. Using high-Throughput RNA-seq, 259 up-regulated transcripts were found, mostly associated with angiogenesis, gluconeogenesis, and triacylglyceride catabolism. Conversely, 293 transcripts were down-regulated, associated to cholesterol biosynthesis, PPARα signaling, fatty acid biosynthesis, and glycolysis. This gene signature was concordant with hepatic metabolite levels and hepatic oxidative damage. Moreover, the increased plasmatic levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase) and AP (alkaline phosphatase), as well as liver histology suggest stress-induced liver steatosis. This study offers an integrative molecular and biochemical analysis of the hepatic response to handling-stress, and reveals unknown aspects of lipid metabolism in a non-model teleost. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0176447 |
| Databáze: | OpenAIRE |
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