DNA Microarray-Mediated Transcriptional Profiling of the Escherichia coli Response to Hydrogen Peroxide
| Autor: | Gisela Storz, Dana R. Smulski, Xunde Wang, Ming Zheng, Lori J. Templeton, Robert A. LaRossa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
DNA
Bacterial Transcription Genetic Operon Molecular Sequence Data Genetics and Molecular Biology Biology medicine.disease_cause Microbiology Regulon chemistry.chemical_compound Bacterial Proteins Gene expression medicine Escherichia coli Hydrogen peroxide Molecular Biology Gene Oligonucleotide Array Sequence Analysis Base Sequence Escherichia coli Proteins Hydrogen Peroxide Molecular biology Cell biology DNA-Binding Proteins Repressor Proteins Response regulator chemistry Trans-Activators bacteria DNA microarray Transcription Factors |
| Popis: | The genome-wide transcription profile of Escherichia coli cells treated with hydrogen peroxide was examined with a DNA microarray composed of 4,169 E. coli open reading frames. By measuring gene expression in isogenic wild-type and oxyR deletion strains, we confirmed that the peroxide response regulator OxyR activates most of the highly hydrogen peroxide-inducible genes. The DNA microarray measurements allowed the identification of several new OxyR-activated genes, including the hemH heme biosynthetic gene; the six-gene suf operon, which may participate in Fe-S cluster assembly or repair; and four genes of unknown function. We also identified several genes, including uxuA, encoding mannonate hydrolase, whose expression might be repressed by OxyR, since their expression was elevated in the Δ oxyR mutant strain. In addition, the induction of some genes was found to be OxyR independent, indicating the existence of other peroxide sensors and regulators in E. coli . For example, the isc operon, which specifies Fe-S cluster formation and repair activities, was induced by hydrogen peroxide in strains lacking either OxyR or the superoxide response regulators SoxRS. These results expand our understanding of the oxidative stress response and raise interesting questions regarding the nature of other regulators that modulate gene expression in response to hydrogen peroxide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|