Role of Free Radical Formation in Murine Cytomegalovirus–Induced Hearing Loss
| Autor: | Taelor Johnson, Pranav Dinesh Mathur, Melissa S. Price, Jun Yang, Phayvanh P. Pecha, Elaine Hillas, Albert H. Park, Namakkal S. Rajasekaran, Ali Almishaal, Matthew A. Firpo, Travis Haller |
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| Rok vydání: | 2020 |
| Předmět: |
Free Radicals
Hearing loss Otoacoustic Emissions Spontaneous Congenital cytomegalovirus infection D methionine Article Mice 03 medical and health sciences 0302 clinical medicine otorhinolaryngologic diseases medicine Animals Outer hair cells Labyrinthitis Free Radical Formation 030304 developmental biology chemistry.chemical_classification Mice Inbred BALB C 0303 health sciences Reactive oxygen species business.industry medicine.disease Molecular biology Disease Models Animal Auditory brainstem response Otorhinolaryngology chemistry Cytomegalovirus Infections Microscopy Electron Scanning Immunohistochemistry Surgery medicine.symptom Reactive Oxygen Species business 030217 neurology & neurosurgery |
| Zdroj: | Otolaryngol Head Neck Surg |
| ISSN: | 1097-6817 0194-5998 |
| DOI: | 10.1177/0194599820901485 |
| Popis: | OBJECTIVES. The goal of the study was to determine whether reactive oxygen species (ROS) mediates cytomegalovirus (CMV)–induced labyrinthitis. STUDY DESIGN. Murine model of CMV infection. SETTING. University of Utah laboratory. SUBJECTS AND METHODS. Nrf2 knockout mice were inoculated with murine CMV. Auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAEs) were then performed on these and uninfected controls. BALB/c mice were inoculated with murine CMV to determine whether a marker for ROS production, dihydroethidium (DHE), is expressed 7 days after inoculation. Finally, 2 antioxidants—D-methionine and ACE-Mg (vitamins A, C, and E with magnesium)—were administered 1 hour before and after infection in inoculated mice for 14 days. Temporal bones were harvested at postnatal day 10 for DHE detection. ABR and DPOAE testing was done at postnatal day 30. Scanning electron microscopy was also performed at postnatal day 30 to evaluate outer hair cell integrity. RESULTS. Nrf2-infected mice had worse hearing than uninfected mice (P < .001). A statistically significant increase in DHE fluorescence was detected in BALB/c-infected mice as compared with uninfected mice 7 days after inoculation. D-methionine- and ACE-Mg-treated mice demonstrated an attenuation of the DHE fluorescence and a significant improvement in ABR and DPOAE thresholds when compared with untreated infected controls (P |
| Databáze: | OpenAIRE |
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